Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Jun;51(6):1224–1229. doi: 10.1128/aem.51.6.1224-1229.1986

Isolation of Halotolerant, Thermotolerant, Facultative Polymer-Producing Bacteria and Characterization of the Exopolymer

S M Pfiffner 1,†, Michael J McInerney 1,*, Gary E Jenneman 1, Roy M Knapp 1
PMCID: PMC239049  PMID: 16347080

Abstract

Over 200 bacterial strains were selected for anaerobic growth at 50°C and extracellular polysaccharide production in a sucrose-mineral salts medium with NaNO3 and up to 10% NaCl. The predominant cell type was an encapsulated gram-positive, motile, facultative sporeforming rod similar to Bacillus species. Strain SP018 grew and produced the polysaccharide on a variety of substrates at salinities up to 12% NaCl. Good polymer production only occurred anaerobically and was optimal between 4 and 10% NaCl. The ethanol-precipitated SP018 polymer was a charged heteropolysaccharide that contained glucose, mannose, arabinose, ribose, and low levels of allose and glucosamine. The SP018 polymer showed pseudoplastic behavior, was resistant to shearing, and had a higher viscosity at dilute concentrations and at elevated temperatures than xanthan gum. High-ionic-strength solutions reversibly decreased the viscosity of SP018 polymer solutions. The bacterium and the associated polymer have many properties that make them potentially useful for in situ microbially enhanced oil recovery processes.

Full text

PDF
1224

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bodie E. A., Schwartz R. D., Catena A. Production and Characterization of a Polymer from Arthrobacter sp. Appl Environ Microbiol. 1985 Sep;50(3):629–633. doi: 10.1128/aem.50.3.629-633.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyle C. D., Reade A. E. Characterization of two extracellular polysaccharides from marine bacteria. Appl Environ Microbiol. 1983 Aug;46(2):392–399. doi: 10.1128/aem.46.2.392-399.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
  5. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  6. Fazio S. A., Uhlinger D. J., Parker J. H., White D. C. Estimations of uronic acids as quantitative measures of extracellular and cell wall polysaccharide polymers from environmental samples. Appl Environ Microbiol. 1982 May;43(5):1151–1159. doi: 10.1128/aem.43.5.1151-1159.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jang L. K., Chang P. W., Findley J. E., Yen T. F. Selection of bacteria with favorable transport properties through porous rock for the application of microbial-enhanced oil recovery. Appl Environ Microbiol. 1983 Nov;46(5):1066–1072. doi: 10.1128/aem.46.5.1066-1072.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Nichols P. D., Henson J. M., Guckert J. B., Nivens D. E., White D. C. Fourier transform-infrared spectroscopic methods for microbial ecology: analysis of bacteria, bacteria-polymer mixtures and biofilms. J Microbiol Methods. 1985;4:79–94. doi: 10.1016/0167-7012(85)90023-5. [DOI] [PubMed] [Google Scholar]
  10. Norberg A. B., Enfors S. O. Production of Extracellular Polysaccharide by Zoogloea ramigera. Appl Environ Microbiol. 1982 Nov;44(5):1231–1237. doi: 10.1128/aem.44.5.1231-1237.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Souw P., Demain A. L. Nutritional Studies on Xanthan Production by Xanthomonas campestris NRRL B1459. Appl Environ Microbiol. 1979 Jun;37(6):1186–1192. doi: 10.1128/aem.37.6.1186-1192.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Williams A. G., Wimpenny J. W. Exopolysaccharide production by Pseudomonas NCIB11264 grown in continuous culture. J Gen Microbiol. 1978 Jan;104(1):47–57. doi: 10.1099/00221287-104-1-47. [DOI] [PubMed] [Google Scholar]
  13. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES