Abstract
The conversion of d-xylose to ethanol by the yeast Pachysolen tannophilus is relatively inefficient in batch culture. The inefficiency has been attributed in part to concurrent utilization of ethanol in the presence of appreciable concentrations of d-xylose and to the formation of xylitol and other by-products. To increase the concentration of ethanol accumulated in batch cultures, UV-induced mutants of P. tannophilus were selected on the basis of diminished growth on ethanol. Eleven independent mutant loci that conferred the ethanol-defective phenotype were identified. Three led to a greater yield and volumetric rate of production of ethanol than the wild type. One also produced less xylitol and was characterized by a deficiency in activity for malate dehydrogenase.
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Selected References
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- BULDER C. J. INDUCTION OF PETITE MUTATION AND INHIBITION OF SYNTHESIS OF RESPIRATORY ENZYMES IN VARIOUS YEASTS. Antonie Van Leeuwenhoek. 1964;30:1–9. doi: 10.1007/BF02046695. [DOI] [PubMed] [Google Scholar]
- Ciriacy M. Isolation and characterization of yeast mutants defective in intermediary carbon metabolism and in carbon catabolite derepression. Mol Gen Genet. 1977 Jul 20;154(2):213–220. doi: 10.1007/BF00330840. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Maleszka R., Schneider H. Concurrent Production and Consumption of Ethanol by Cultures of Pachysolen tannophilus Growing on d-Xylose. Appl Environ Microbiol. 1982 Oct;44(4):909–912. doi: 10.1128/aem.44.4.909-912.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maleszka R., Schneider H. Fermentation of D-xylose, xylitol, and D-xylulose by yeasts. Can J Microbiol. 1982 Mar;28(3):360–363. doi: 10.1139/m82-054. [DOI] [PubMed] [Google Scholar]
- Neirinck L. G., Maleszka R., Schneider H. The requirement of oxygen for incorporation of carbon from D-xylose and D-glucose by Pachysolen tannophilus. Arch Biochem Biophys. 1984 Jan;228(1):13–21. doi: 10.1016/0003-9861(84)90041-9. [DOI] [PubMed] [Google Scholar]
- OGUR M., ROSHANMANESH A., OGUR S. TRICARBOXYLIC ACID CYCLE MUTANTS IN SACCHAROMYCES: COMPARISON OF INDEPENDENTLY DERIVED MUTANTS. Science. 1965 Mar 26;147(3665):1590–1590. doi: 10.1126/science.147.3665.1590. [DOI] [PubMed] [Google Scholar]
- Toivola A., Yarrow D., van den Bosch E., van Dijken J. P., Scheffers W. A. Alcoholic Fermentation of d-Xylose by Yeasts. Appl Environ Microbiol. 1984 Jun;47(6):1221–1223. doi: 10.1128/aem.47.6.1221-1223.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]