Abstract
Isotopic tracer experiments were conducted in vitro to determine contribution of protozoa toward the biosynthesis of lysine in the rumen microbial ecosystem. The presence of protozoa in a rumen microbial suspension always increased lysine synthesis from aspartate. Rumen contents from a faunated goat produced a higher amount of lysine than did those from a defaunated one.
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Selected References
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- Akkada A. R., Bartley E. E., Berube R., Fina L. R., Meyer R. M., Henricks D., Julius F. Simple method of remove completely ciliate protozoa of adult ruminants. Appl Microbiol. 1968 Oct;16(10):1475–1477. doi: 10.1128/am.16.10.1475-1477.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DARROCH R. A., EDWARDS D. C. The synthesis of lysine by the lactating goat. Br J Nutr. 1956;10(4):286–292. doi: 10.1079/bjn19560044. [DOI] [PubMed] [Google Scholar]
- Emmanuel B., Milligan L. P., Turner B. V. The metabolism of acetate by rumen microorganisms. Can J Microbiol. 1974 Feb;20(2):183–185. doi: 10.1139/m74-028. [DOI] [PubMed] [Google Scholar]
- Kurihara Y., Eadie J. M., Hobson P. N., Mann S. O. Relationship between bacteria and ciliate protozoa in the sheep rumen. J Gen Microbiol. 1968 Apr;51(2):267–288. doi: 10.1099/00221287-51-2-267. [DOI] [PubMed] [Google Scholar]
- Loosli J. K., Williams H. H., Thomas W. E., Ferris F. H., Maynard L. A. Synthesis of Amino Acids in the Rumen. Science. 1949 Aug 5;110(2849):144–145. doi: 10.1126/science.110.2849.144. [DOI] [PubMed] [Google Scholar]
- Onodora R., Kandatsu M. Synthesis of lysine from alpha, epsilon-diaminopimelic acid by mixed ciliated rumen protozoa. Nat New Biol. 1973 Jul 4;244(131):31–32. doi: 10.1038/newbio244031a0. [DOI] [PubMed] [Google Scholar]
- Rowe J. B., Davies A., Broome A. W. Quantitative effects of defaunation on rumen fermentation and digestion in sheep. Br J Nutr. 1985 Jul;54(1):105–119. doi: 10.1079/bjn19850097. [DOI] [PubMed] [Google Scholar]
- Sauer F. D., Erfle J. D., Mahadevan S. Amino acid biosynthesis in mixed rumen cultures. Biochem J. 1975 Sep;150(3):357–372. doi: 10.1042/bj1500357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Virtanen A. I. Milk production of cows on protein-free feed. Science. 1966 Sep 30;153(3744):1603–1614. doi: 10.1126/science.153.3744.1603. [DOI] [PubMed] [Google Scholar]
