Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Jun;51(6):1361–1363. doi: 10.1128/aem.51.6.1361-1363.1986

New, simple medium for selective, differential recovery of Klebsiella spp.

J M Tomás, B Ciurana, J T Jofre
PMCID: PMC239073  PMID: 3729402

Abstract

A highly selective, differential medium for the enumeration and isolation of Klebsiella spp. was developed. With pure cultures, 100% recovery of Klebsiella spp. was observed. Recovery of Klebsiella spp. on MacConkey-inositol-potassium tellurite (MCIK) agar was as good as or better than on MacConkey-inositol-carbenicillin agar either with pure cultures or environmental samples. Recovery and percent colony confirmation with MCIK agar were greater and easier to obtain than for other proposed Klebsiella selective media.

Full text

PDF
1361

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bagley S. T., Seidler R. J. Primary Klebsiella identification with MacConkey-inositol-carbenicillin agar. Appl Environ Microbiol. 1978 Sep;36(3):536–538. doi: 10.1128/aem.36.3.536-538.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bagley S. T., Seidler R. J. Significance of fecal coliform-positive Klebsiella. Appl Environ Microbiol. 1977 May;33(5):1141–1148. doi: 10.1128/aem.33.5.1141-1148.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Matsen J. M., Spindler J. A., Blosser R. O. Characterization of Klebsiella isolates from natural receiving waters and comparison with human isolates. Appl Microbiol. 1974 Oct;28(4):672–678. doi: 10.1128/am.28.4.672-678.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Naemura L. G., Seidler R. J. Significance of low-temperature growth associated with the fecal coliform response, indole production, and pectin liquefaction in Klebsiella. Appl Environ Microbiol. 1978 Feb;35(2):392–396. doi: 10.1128/aem.35.2.392-396.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Seidler R. J., Morrow J. E., Bagley S. T. Klebsielleae in drinking water emanating from redwood tanks. Appl Environ Microbiol. 1977 Apr;33(4):893–900. doi: 10.1128/aem.33.4.893-900.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Smith H. W. Arsenic resistance in enterobacteria: its transmission by conjugation and by phage. J Gen Microbiol. 1978 Nov;109(1):49–56. doi: 10.1099/00221287-109-1-49. [DOI] [PubMed] [Google Scholar]
  8. Summers A. O., Jacoby G. A. Plasmid-determined resistance to tellurium compounds. J Bacteriol. 1977 Jan;129(1):276–281. doi: 10.1128/jb.129.1.276-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Taylor D. E., Summers A. O. Association of tellurium resistance and bacteriophage inhibition conferred by R plasmids. J Bacteriol. 1979 Mar;137(3):1430–1433. doi: 10.1128/jb.137.3.1430-1433.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Thom B. T. Klebsiella in faeces. Lancet. 1970 Nov 14;2(7681):1033–1033. doi: 10.1016/s0140-6736(70)92845-x. [DOI] [PubMed] [Google Scholar]
  11. Tomás J., Regué M., Parés R., Jofre J., Kay W. W. P1 bacteriophage and tellurite sensitivity in Klebsiella pneumoniae and Escherichia coli. Can J Microbiol. 1984 Jun;30(6):830–836. doi: 10.1139/m84-127. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES