Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Jun;51(6):1367–1369. doi: 10.1128/aem.51.6.1367-1369.1986

Isolation of Endoglucanase Genes from Pseudomonas fluorescens subsp. cellulosa and a Pseudomonas sp

Bruce R Wolff 1, Terry A Mudry 1, Bernard R Glick 1, J J Pasternak 1,*
PMCID: PMC239075  PMID: 16347092

Abstract

Endoglucanase genes from Pseudomonas fluorescens subsp. cellulosa and Pseudomonas sp. were cloned and characterized. DNA hybridization studies showed that these genes are homologous and that each species has one copy of the gene per genome. The DNA fragment from Pseudomonas sp. codes for, at most, a 23-kilodalton endoglucanase.

Full text

PDF
1367

Images in this article

1368Image
on p.1368
1368Image
on p.1368

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armentrout R. W., Brown R. D. Molecular cloning of genes for cellobiose utilization and their expression in Escherichia coli. Appl Environ Microbiol. 1981 Jun;41(6):1355–1362. doi: 10.1128/aem.41.6.1355-1362.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Béguin P., Cornet P., Aubert J. P. Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum. J Bacteriol. 1985 Apr;162(1):102–105. doi: 10.1128/jb.162.1.102-105.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chahal D. S. Solid-State Fermentation with Trichoderma reesei for Cellulase Production. Appl Environ Microbiol. 1985 Jan;49(1):205–210. doi: 10.1128/aem.49.1.205-210.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kohchi C., Toh-e A. Nucleotide sequence of Candida pelliculosa beta-glucosidase gene. Nucleic Acids Res. 1985 Sep 11;13(17):6273–6282. doi: 10.1093/nar/13.17.6273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sashihara N., Kudo T., Horikoshi K. Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli. J Bacteriol. 1984 May;158(2):503–506. doi: 10.1128/jb.158.2.503-506.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stewart B. J., Leatherwood J. M. Derepressed synthesis of cellulase by Cellulomonas. J Bacteriol. 1976 Nov;128(2):609–615. doi: 10.1128/jb.128.2.609-615.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Teather R. M., Wood P. J. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol. 1982 Apr;43(4):777–780. doi: 10.1128/aem.43.4.777-780.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Whittle D. J., Kilburn D. G., Warren R. A., Miller R. C., Jr Molecular cloning of a Cellulomonas fimi cellulose gene in Escherichia coli. Gene. 1982 Feb;17(2):139–145. doi: 10.1016/0378-1119(82)90066-x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES