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. 1986 Oct;52(4):660–664. doi: 10.1128/aem.52.4.660-664.1986

Affinity Purifications of Aldose Reductase and Xylitol Dehydrogenase from the Xylose-Fermenting Yeast Pachysolen tannophilus

Paul L Bolen 1,*, Kelly A Roth 1, Shelby N Freer 1
PMCID: PMC239093  PMID: 16347161

Abstract

Although xylose is a major product of hydrolysis of lignocellulosic materials, few yeasts are able to convert it to ethanol. In Pachysolen tannophilus, one of the few xylose-fermenting yeasts found, aldose reductase and xylitol dehydrogenase were found to be key enzymes in the metabolic pathway for xylose fermentation. This paper presents a method for the rapid and simultaneous purification of both aldose reductase and xylitol dehydrogenase from P. tannophilus. Preliminary studies indicate that this method may be easily adapted to purify similar enzymes from other xylose-fermenting yeasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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