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. 1986 Oct;52(4):706–710. doi: 10.1128/aem.52.4.706-710.1986

Purification and Biochemical Analysis of the Cytoplasmic Membrane from the Desiccation-Tolerant Cyanobacterium Nostoc commune UTEX 584

Jaap J Olie 2, Malcolm Potts 2,*
PMCID: PMC239101  PMID: 16347165

Abstract

The cytoplasmic membrane of the heterocystous cyanobacterium Nostoc commune UTEX 584 was isolated free of thylakoids and phycobiliprotein-membrane complexes by flotation centrifugation. Purified membranes had a buoyant density of 1.07 g cm−3 and were bright orange. Twelve major proteins were detected in the membrane, and of these, the most abundant had molecular masses of 83, 71, 68, 51, and 46 kilodaltons. The ester-linked fatty acids of the methanol fraction contained 16:0, 18:0, 18:1ω9c, 20:0, and 20:3ω3 with no traces of hydroxy fatty acids. Compound 20:3ω3 represented 56.8% of the total fatty acid methyl esters, a feature which distinguishes the cell membrane of N. commune UTEX 584 from those of all other cyanobacteria which have been characterized to date. Fatty acid 18:3 was not detected. Carotenoids were analyzed by highperformance liquid chromatography. The cytoplasmic membrane contained β-carotene and echinenone as the dominant carotenoids and lacked chlorophyll a and pheophytin a. Whole cells contained β-carotene and echinenone, and lesser amounts of zeaxanthin and (3R)-cryptoxanthin.

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Selected References

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  1. Biel A. J., Marrs B. L. Oxygen does not directly regulate carotenoid biosynthesis in Rhodopseudomonas capsulata. J Bacteriol. 1985 Jun;162(3):1320–1321. doi: 10.1128/jb.162.3.1320-1321.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Collins M. L., Niederman R. A. Membranes of Rhodospirillum rubrum: isolation and physicochemical properties of membranes from aerobically grown cells. J Bacteriol. 1976 Jun;126(3):1316–1325. doi: 10.1128/jb.126.3.1316-1325.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Holton R. W., Blecker H. H., Stevens T. S. Fatty acids in blue-green algae: possible relation to phylogenetic position. Science. 1968 May 3;160(3827):545–547. doi: 10.1126/science.160.3827.545. [DOI] [PubMed] [Google Scholar]
  4. Jürgens U. J., Weckesser J. Carotenoid-containing outer membrane of Synechocystis sp. strain PCC6714. J Bacteriol. 1985 Oct;164(1):384–389. doi: 10.1128/jb.164.1.384-389.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kenyon C. N., Rippka R., Stanier R. Y. Fatty acid composition and physiological properties of some filamentous blue-green algae. Arch Mikrobiol. 1972;83(3):216–236. doi: 10.1007/BF00645123. [DOI] [PubMed] [Google Scholar]
  6. Kenyon C. N., Stanier R. Y. Possible evolutionary significance of polyunsaturated fatty acids in blue-green algae. Nature. 1970 Sep 12;227(5263):1164–1166. doi: 10.1038/2271164a0. [DOI] [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  9. Potts M. Protein synthesis and proteolysis in immobilized cells of the cyanobacterium Nostoc commune UTEX 584 exposed to matric water stress. J Bacteriol. 1985 Dec;164(3):1025–1031. doi: 10.1128/jb.164.3.1025-1031.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schnaitman C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J Bacteriol. 1970 Nov;104(2):890–901. doi: 10.1128/jb.104.2.890-901.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stransky H., Hager A. Das Carotinoidmuster und die Verbreitung des lichtinduzierten Xanthophyllcyclus in verschiedenen Algenklassen. IV. Cyanophyceae und Rhodophyceae. Arch Mikrobiol. 1970;72(1):84–96. [PubMed] [Google Scholar]
  12. Vaara T., Vaara M., Niemelä S. Two improved methods for obtaining axenic cultures of cyanobacteria. Appl Environ Microbiol. 1979 Nov;38(5):1011–1014. doi: 10.1128/aem.38.5.1011-1014.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Varga A. R., Staehelin L. A. Pigment-protein complexes from Rhodopseudomonas palustris: isolation, characterization, and reconstitution into liposomes. J Bacteriol. 1985 Mar;161(3):921–927. doi: 10.1128/jb.161.3.921-927.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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