Abstract
Acremonium coenophialum, an endophytic fungus present in toxic tall fescue grass and seed, grew very slowly or not at all with conventional media and cultural practices. However, a considerable increase in growth was achieved in a relatively dilute medium consisting solely of glucose and yeast extract. The optimal levels of glucose and yeast extract were 3 to 6% and 0.35% (wt/vol), respectively. The addition of salts which lowered the pH suppressed growth. Even when the pH was controlled, the addition of KH2PO4 at a level of 3.2% or more greatly inhibited growth. A. coenophialum grew better in shake culture than in stationary culture. The optimal temperature was 23°C, and the optimal pH was 6.5.
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Selected References
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- Bacon C. W., Porter J. K., Robbins J. D., Luttrell E. S. Epichloë typhina from toxic tall fescue grasses. Appl Environ Microbiol. 1977 Nov;34(5):576–581. doi: 10.1128/aem.34.5.576-581.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis N. D., Diener U. L., Eldridge D. W. Production of aflatoxins B1 and G1 by Aspergillus flavus in a semisynthetic medium. Appl Microbiol. 1966 May;14(3):378–380. doi: 10.1128/am.14.3.378-380.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter J. K., Bacon C. W., Robbins J. D. Ergosine, ergosinine, and chanoclavine I from Epichloë typhina. J Agric Food Chem. 1979 May-Jun;27(3):595–598. doi: 10.1021/jf60223a045. [DOI] [PubMed] [Google Scholar]