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. 1986 Oct;52(4):924–929. doi: 10.1128/aem.52.4.924-929.1986

Isolation and Characterization of Linear DNA Elements from the Mitochondria of Gaeumannomyces graminis

Allen L Honeyman 1,, Thomas C Currier 1,§,*
PMCID: PMC239139  PMID: 16347184

Abstract

Different Gaeumannomyces graminis strains of diverse geographic origin contain one or two small DNAs ranging in size from 7.2 to 10 kilobases. These DNAs exhibit different degrees of homology with each other. We have characterized these low-molecular-weight DNAs from one strain, Ha-01. These small DNAs, E1 and E2, are mitochondrial in origin and were isolated as linear molecules which exhibited an intrinsic difference in density from the high-molecular-weight DNA.

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Selected References

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  1. Collins R. A., Stohl L. L., Cole M. D., Lambowitz A. M. Characterization of a novel plasmid DNA found in mitochondria of N. crassa. Cell. 1981 May;24(2):443–452. doi: 10.1016/0092-8674(81)90335-4. [DOI] [PubMed] [Google Scholar]
  2. Cummings D. J., Belcour L., Grandchamp C. Mitochondrial DNA from Podospora anserina. I. Isolation and characterization. Mol Gen Genet. 1979 Mar 27;171(3):229–238. doi: 10.1007/BF00267577. [DOI] [PubMed] [Google Scholar]
  3. Hensgens L. A., Grivell L. A., Borst P., Bos J. L. Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1663–1667. doi: 10.1073/pnas.76.4.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kemble R. J., Mans R. J. Examination of the mitochondrial genome of revertant progeny from S cms maize with cloned S-1 and S-2 hybridization probes. J Mol Appl Genet. 1983;2(2):161–171. [PubMed] [Google Scholar]
  5. Kemble R. J., Thompson R. D. S1 and S2, the linear mitochondrial DNAs present in a male sterile line of maize, possess terminally attached proteins. Nucleic Acids Res. 1982 Dec 20;10(24):8181–8190. doi: 10.1093/nar/10.24.8181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pritchard A. E., Cummings D. J. Replication of linear mitochondrial DNA from Paramecium: sequence and structure of the initiation-end crosslink. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7341–7345. doi: 10.1073/pnas.78.12.7341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rekosh D. M., Russell W. C., Bellet A. J., Robinson A. J. Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977 Jun;11(2):283–295. doi: 10.1016/0092-8674(77)90045-9. [DOI] [PubMed] [Google Scholar]
  8. Stahl U., Kück U., Tudzynski P., Esser K. Characterization and cloning of plasmid like DNA of the ascomycete Podospora anserina. Mol Gen Genet. 1980;178(3):639–646. doi: 10.1007/BF00337872. [DOI] [PubMed] [Google Scholar]
  9. Thompson R. D., Kemble R. J., Flavell R. B. Variations in mitochondrial DNA organisation between normal and male-sterile cytoplasms of maize. Nucleic Acids Res. 1980 May 10;8(9):1999–2008. doi: 10.1093/nar/8.9.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vierny C., Keller A. M., Begel O., Belcour L. A sequence of mitochondrial DNA is associated with the onset of senescence in a fungus. Nature. 1982 May 13;297(5862):157–159. doi: 10.1038/297157a0. [DOI] [PubMed] [Google Scholar]

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