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. 1986 Oct;52(4):969–970. doi: 10.1128/aem.52.4.969-970.1986

Evaluation of the diagnostic application of an enzyme immunoassay for Clostridium perfringens type A enterotoxin.

S G Jackson, D A Yip-Chuck, M H Brodsky
PMCID: PMC239152  PMID: 2877617

Abstract

The diagnostic application of an enzyme immunoassay for Clostridium perfringens type A enterotoxin was evaluated. Test results from 100 individuals associated with C. perfringens gastroenteritis outbreaks and 111 control individuals were included. The assay sensitivity was 93.7%, and the assay specificity was 98.7%.

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Selected References

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  1. Bartholomew B. A., Stringer M. F., Watson G. N., Gilbert R. J. Development and application of an enzyme linked immunosorbent assay for Clostridium perfringens type A enterotoxin. J Clin Pathol. 1985 Feb;38(2):222–228. doi: 10.1136/jcp.38.2.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hauschild A. H. Criteria and procedures for implicating Clostridium perfringens in food-borne outbreaks. Can J Public Health. 1975 Sep-Oct;66(5):388–392. [PubMed] [Google Scholar]
  3. Jackson S. G., Yip-Chuck D. A., Brodsky M. H. A double antibody sandwich enzyme-immunoassay for Clostridium perfringens type A enterotoxin detection in stool specimens. J Immunol Methods. 1985 Oct 24;83(1):141–150. doi: 10.1016/0022-1759(85)90067-5. [DOI] [PubMed] [Google Scholar]
  4. Jackson S. G., Yip-Chuck D. A., Clark J. B., Brodsky M. H. Diagnostic importance of Clostridium perfringens enterotoxin analysis in recurring enteritis among elderly, chronic care psychiatric patients. J Clin Microbiol. 1986 Apr;23(4):748–751. doi: 10.1128/jcm.23.4.748-751.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McClane B. A., Strouse R. J. Rapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay. J Clin Microbiol. 1984 Feb;19(2):112–115. doi: 10.1128/jcm.19.2.112-115.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Olsvik O., Granum P. E., Berdal B. P. Detection of Clostridium perfringens type A enterotoxin by ELISA. Acta Pathol Microbiol Immunol Scand B. 1982 Dec;90(6):445–447. doi: 10.1111/j.1699-0463.1982.tb00144.x. [DOI] [PubMed] [Google Scholar]
  7. Schiemann D. A. Laboratory confirmation of an outbreak of Clostridium perfringens food poisoning. Health Lab Sci. 1977 Jan;14(1):35–38. [PubMed] [Google Scholar]
  8. Sutton R. G., Hobbs B. C. Food poisoning caused by heat-sensitive Clostridium welchii. A report of five recent outbreaks. J Hyg (Lond) 1968 Mar;66(1):135–146. doi: 10.1017/s0022172400041000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Viscidi R., Laughon B. E., Hanvanich M., Bartlett J. G., Yolken R. H. Improved enzyme immunoassays for the detection of antigens in fecal specimens. Investigation and correction of interfering factors. J Immunol Methods. 1984 Feb 24;67(1):129–143. doi: 10.1016/0022-1759(84)90092-9. [DOI] [PubMed] [Google Scholar]
  10. Yamagishi T., Serikawa T., Morita R., Nakamura S., Nishida S. Persistent high numbers of Clostridium perfringens in the intestines of Japanese aged adults. Jpn J Microbiol. 1976 Oct;20(5):397–403. doi: 10.1111/j.1348-0421.1976.tb01005.x. [DOI] [PubMed] [Google Scholar]
  11. Yamagishi T., Serikawa T., Morita R., Takahashi K., Nishida S. Effect of a lactobacillus product administration on the anaerobic intestinal flora of aged adults. Jpn J Microbiol. 1974 May;18(3):211–216. doi: 10.1111/j.1348-0421.1974.tb00947.x. [DOI] [PubMed] [Google Scholar]
  12. Yolken R. H., Stopa P. J. Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus. J Clin Microbiol. 1979 Nov;10(5):703–707. doi: 10.1128/jcm.10.5.703-707.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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