Abstract
Pure cultures of a gram-negative, rod-shaped bacterium, which produced endospores after 3 to 5 days on solid medium, were isolated exclusively from tissue cultures of the date palm Phoenix dactylifera L. Electron microscopic examination of thin sections of the bacteria revealed the bilayer membrane typical of gramnegative bacteria and confirmed the nature of the spores as true endospores. Biochemical and physiological tests indicated that the bacteria were Bacillus circulans. B. circulans was consistently isolated from the internal tissues, including the meristem, of apparently healthy offshoots of date palm. When meristem and embryo callus tissue culture samples were injected with B. circulans isolated from similar tissue culture samples and from offshoots, the majority of the isolates produced a rapid, destructive soft rot of the tissues.
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- BLENDEN D. C., GOLDBERG H. S. SILVER IMPREGNATION STAIN FOR LEPTOSPIRA AND FLAGELLA. J Bacteriol. 1965 Mar;89:899–900. doi: 10.1128/jb.89.3.899-900.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mundt J. O., Hinkle N. F. Bacteria within ovules and seeds. Appl Environ Microbiol. 1976 Nov;32(5):694–698. doi: 10.1128/aem.32.5.694-698.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Barjac H., Bonnefoi A. Essai de classification biochimique de 64 Bacillus des groupes 2 et 3 représentant 11 espèces différentes. Ann Inst Pasteur (Paris) 1972 Mar;122(3):463–473. [PubMed] [Google Scholar]