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. 1986 Nov;52(5):1206–1208. doi: 10.1128/aem.52.5.1206-1208.1986

Thymidine Incorporation by the Microbial Community of Standing Dead Spartina alterniflora†

Robert D Fallon 1,*, Steven Y Newell 1
PMCID: PMC239199  PMID: 16347220

Abstract

Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of [methly-3H]thymidine were nonsaturable over a wide concentration range (101 to 105 nM). Owing to metabolism by both fungi and bacteria, a major fraction of the radiolabel (mean, 48%) appeared in protein. Extraction of the radiolabeled macromolecules was inefficient, averaging 8.8%. Based on an empirically derived conversion factor, 4 × 1018 cells · mol of thymidine−1, doubling times ranged from 4 to 69 h for the epiphytic bacterial assemblage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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