Abstract
Of 15 Vibrio cholerae non-O1 strains, 2 produced a hemolysin termed NAG-rTDH, which is very similar to the thermostable direct hemolysin of V. parahaemolyticus. These two strains contained DNA sequences which are homologous to a DNA probe for the V. parahaemolyticus thermostable direct hemolysin gene. A probe-positive 9-kilobase HindIII fragment was cloned from a plasmid of a V. cholerae non-O1 strain into plasmid pBR322, and the resulting Escherichia coli clones produced intracellular NAG-rTDH.
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- Arita M., Takeda T., Honda T., Miwatani T. Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin. Infect Immun. 1986 Apr;52(1):45–49. doi: 10.1128/iai.52.1.45-49.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blake P. A., Weaver R. E., Hollis D. G. Diseases of humans (other than cholera) caused by vibrios. Annu Rev Microbiol. 1980;34:341–367. doi: 10.1146/annurev.mi.34.100180.002013. [DOI] [PubMed] [Google Scholar]
- Craig J. P., Yamamoto K., Takeda Y., Miwatani T. Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 strain isolated from the environment. Infect Immun. 1981 Oct;34(1):90–97. doi: 10.1128/iai.34.1.90-97.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Arita M., Takeda T., Yoh M., Miwatani T. Non-O1 Vibrio cholerae produces two newly identified toxins related to Vibrio parahaemolyticus haemolysin and Escherichia coli heat-stable enterotoxin. Lancet. 1985 Jul 20;2(8447):163–164. doi: 10.1016/s0140-6736(85)90277-6. [DOI] [PubMed] [Google Scholar]
- Honda T., Yoh M., Kongmuang U., Miwatani T. Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus. J Clin Microbiol. 1985 Sep;22(3):383–386. doi: 10.1128/jcm.22.3.383-386.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaper J. B., Campen R. K., Seidler R. J., Baldini M. M., Falkow S. Cloning of the thermostable direct or Kanagawa phenomenon-associated hemolysin of Vibrio parahaemolyticus. Infect Immun. 1984 Jul;45(1):290–292. doi: 10.1128/iai.45.1.290-292.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris J. G., Jr, Black R. E. Cholera and other vibrioses in the United States. N Engl J Med. 1985 Feb 7;312(6):343–350. doi: 10.1056/NEJM198502073120604. [DOI] [PubMed] [Google Scholar]
- Nishibuchi M., Ishibashi M., Takeda Y., Kaper J. B. Detection of the thermostable direct hemolysin gene and related DNA sequences in Vibrio parahaemolyticus and other vibrio species by the DNA colony hybridization test. Infect Immun. 1985 Sep;49(3):481–486. doi: 10.1128/iai.49.3.481-486.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishibuchi M., Kaper J. B. Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio parahaemolyticus. J Bacteriol. 1985 May;162(2):558–564. doi: 10.1128/jb.162.2.558-564.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishibuchi M., Seidler R. J. Medium-dependent production of extracellular enterotoxins by non-O-1 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis. Appl Environ Microbiol. 1983 Jan;45(1):228–231. doi: 10.1128/aem.45.1.228-231.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishibuchi M., Seidler R. J., Rollins D. M., Joseph S. W. Vibrio factors cause rapid fluid accumulation in suckling mice. Infect Immun. 1983 Jun;40(3):1083–1091. doi: 10.1128/iai.40.3.1083-1091.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seidler R. J., Mandel M. Quantitative aspects of deoxyribonucleic acid renaturation: base composition, state of chromosome replication, and polynucleotide homologies. J Bacteriol. 1971 May;106(2):608–614. doi: 10.1128/jb.106.2.608-614.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol. 1979;68:152–176. doi: 10.1016/0076-6879(79)68011-4. [DOI] [PubMed] [Google Scholar]
- Yamamoto K., Al-Omani M., Honda T., Takeda Y., Miwatani T. Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin. Infect Immun. 1984 Jul;45(1):192–196. doi: 10.1128/iai.45.1.192-196.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yamamoto K., Takeda Y., Miwatani T., Craig J. P. Evidence that a non-O1 Vibrio cholerae produces enterotoxin that is similar but not identical to cholera enterotoxin. Infect Immun. 1983 Sep;41(3):896–901. doi: 10.1128/iai.41.3.896-901.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoh M., Honda T., Miwatani T. Purification and partial characterization of a non-O1 Vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of Vibrio parahaemolyticus. Infect Immun. 1986 Apr;52(1):319–322. doi: 10.1128/iai.52.1.319-322.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zinnaka Y., Carpenter C. C., Jr An enterotoxin produced by noncholera vibrios. Johns Hopkins Med J. 1972 Dec;131(6):403–411. [PubMed] [Google Scholar]



