Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Nov;52(5):1227–1229. doi: 10.1128/aem.52.5.1227-1229.1986

Isolation and Characterization of a Fast-Growing, Thermophilic Methanobacterium Species

Yizhang Zhao 2, Hui Zhang 2, David R Boone 2,*, Robert A Mah 2
PMCID: PMC239206  PMID: 16347224

Abstract

A thermophilic, autotrophic methanogen (strain CB12, DSM 3664) was isolated from a mesophilic biogas digestor. This bacterium used H2-CO2 or formate as a substrate and grew as short rods, sometimes in pairs and in crooked filaments. Motility was not observed. Its optimum temperature (56°C) was lower than that of other thermophilic members of the genus Methanobacterium. The maximum observed specific growth rate was 0.564 h−1 (74-min doubling time).

Full text

PDF
1227

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Schauer N. L., Ferry J. G. Metabolism of formate in Methanobacterium formicicum. J Bacteriol. 1980 Jun;142(3):800–807. doi: 10.1128/jb.142.3.800-807.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. WOLIN E. A., WOLIN M. J., WOLFE R. S. FORMATION OF METHANE BY BACTERIAL EXTRACTS. J Biol Chem. 1963 Aug;238:2882–2886. [PubMed] [Google Scholar]
  3. Zeikus J. G., Wolfe R. S. Fine structure of Methanobacterium thermoautotrophicum: effect of growth temperature on morphology and ultrastructure. J Bacteriol. 1973 Jan;113(1):461–467. doi: 10.1128/jb.113.1.461-467.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Zeikus J. G., Wolfe R. S. Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J Bacteriol. 1972 Feb;109(2):707–715. doi: 10.1128/jb.109.2.707-713.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES