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. 1986 Dec;52(6):1382–1385. doi: 10.1128/aem.52.6.1382-1385.1986

Cloning and expression of a Streptomyces cholesterol oxidase gene in Streptomyces lividans with plasmid pIJ702.

Y Murooka, T Ishizaki, O Nimi, N Maekawa
PMCID: PMC239237  PMID: 3466572

Abstract

The cholesterol oxidase gene (cho) of Streptomyces sp. was cloned into Streptomyces lividans with the vector pIJ702. Deletion analysis of the recombinant plasmid showed that entire coding sequence of the cho gene was located within a 2.5-kilobase segment of the chromosomal DNA obtained from the cholesterol oxidase-producing strain. When cloned cells of S. lividans were grown in an appropriate medium, the cells produced severalfold more cholesterol oxidase extracellularly than did the producing strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allain C. C., Poon L. S., Chan C. S., Richmond W., Fu P. C. Enzymatic determination of total serum cholesterol. Clin Chem. 1974 Apr;20(4):470–475. [PubMed] [Google Scholar]
  2. Fukuyama M., Miyake Y. Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin. J Biochem. 1979 May;85(5):1183–1193. [PubMed] [Google Scholar]
  3. Gray G., Selzer G., Buell G., Shaw P., Escanez S., Hofer S., Voegeli P., Thompson C. J. Synthesis of bovine growth hormone by Streptomyces lividans. Gene. 1984 Dec;32(1-2):21–30. doi: 10.1016/0378-1119(84)90028-3. [DOI] [PubMed] [Google Scholar]
  4. Kamei T., Takiguchi Y., Suzuki H., Matsuzaki M., Nakamura S. Purification of 3beta-hydroxysteroid oxidase of Streptomyces violascens origin by affinity chromatography on cholesterol. Chem Pharm Bull (Tokyo) 1978 Sep;26(9):2799–2804. doi: 10.1248/cpb.26.2799. [DOI] [PubMed] [Google Scholar]
  5. Katz E., Thompson C. J., Hopwood D. A. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol. 1983 Sep;129(9):2703–2714. doi: 10.1099/00221287-129-9-2703. [DOI] [PubMed] [Google Scholar]
  6. Kenney W. C., Singer T. P., Fukuyama M., Miyake Y. Identification of the covalently bound flavin prosthetic group of cholesterol oxidase. J Biol Chem. 1979 Jun 10;254(11):4689–4690. [PubMed] [Google Scholar]
  7. Kieser T., Hopwood D. A., Wright H. M., Thompson C. J. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet. 1982;185(2):223–228. doi: 10.1007/BF00330791. [DOI] [PubMed] [Google Scholar]
  8. Muñoz A., Perez-Aranda A., Barbero J. L. Cloning and expression of human interleukin 2 in Streptomyces lividans using the Escherichia coli consensus promoter. Biochem Biophys Res Commun. 1985 Dec 17;133(2):511–519. doi: 10.1016/0006-291x(85)90936-2. [DOI] [PubMed] [Google Scholar]
  9. STADTMAN T. C., CHERKES A., ANFINSEN C. B. Studies on the microbiological degradation of cholesterol. J Biol Chem. 1954 Feb;206(2):511–523. [PubMed] [Google Scholar]
  10. Schottel J. L., Bibb M. J., Cohen S. N. Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli. J Bacteriol. 1981 Apr;146(1):360–368. doi: 10.1128/jb.146.1.360-368.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sugiyama M., Mochizuki H., Nimi O., Nomi R. Mechanism of protection of protein synthesis against streptomycin inhibition in a producing strain. J Antibiot (Tokyo) 1981 Sep;34(9):1183–1188. doi: 10.7164/antibiotics.34.1183. [DOI] [PubMed] [Google Scholar]
  12. Takizawa N., Murooka Y. Cloning of the pullulanase gene and overproduction of pullulanase in Escherichia coli and Klebsiella aerogenes. Appl Environ Microbiol. 1985 Feb;49(2):294–298. doi: 10.1128/aem.49.2.294-298.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thompson C. J., Ward J. M., Hopwood D. A. Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol. 1982 Aug;151(2):668–677. doi: 10.1128/jb.151.2.668-677.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tomioka H., Kagawa M., Nakamura S. Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens. J Biochem. 1976 May;79(5):903–915. doi: 10.1093/oxfordjournals.jbchem.a131158. [DOI] [PubMed] [Google Scholar]

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