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. 1986 Dec;52(6):1403–1406. doi: 10.1128/aem.52.6.1403-1406.1986

Microbial Oxidation of Hydrocarbons and Related Compounds by Whole-Cell Suspensions of the Methane-Oxidizing Bacterium H-2

Takeo Imai 1, Hirofumi Takigawa 1, Satoshi Nakagawa 1, Gwo-Jenn Shen 1, Tohru Kodama 1,*, Yasuji Minoda 1
PMCID: PMC239242  PMID: 16347244

Abstract

Previously, a thermophilic obligate methane-oxidizing bacterium, H-2 (type I), was isolated in our laboratory. H-2 is a new type of methylotroph because of the G+C content of DNA; it uses both the ribulose monophosphate pathway and the serine pathway for carbon assimilation and possesses a new quinone. In addition, we found that resting cell suspensions of H-2 had the ability to oxidize a variety of compounds different from the other methane-oxidizing bacteria as follows. (i) C1 to C8n-alkanes are hydroxylated and further oxidized, yielding mixtures of the corresponding alcohols, aldehydes, acids, and ketones. Liquid alkanes are transformed through a different oxidative pathway from that of gaseous ones. (ii) Both gaseous (C2 to C4) and liquid (C5, C6) n-alkenes are oxidized to their corresponding 1,2-epoxides. (iii) Liquid monochloro and dichloro n-alkanes (C5, C6) are oxidized, yielding their corresponding acids or haloacids. (iv) Diethyl ether is oxidized to acetic acid; no ethanol and acetaldehyde are detected. (v) Cyclic and aromatic compounds are also oxidized. (vi) Secondary alcohols (C3 to C10) are oxidized to their corresponding methyl ketones.

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Selected References

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  1. Abbott B. J., Hou C. T. Oxidation of 1-alkenes to 1,2-epoxyalkanes by Pseudomonas oleovorans. Appl Microbiol. 1973 Jul;26(1):86–91. doi: 10.1128/am.26.1.86-91.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Colby J., Stirling D. I., Dalton H. The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds. Biochem J. 1977 Aug 1;165(2):395–402. doi: 10.1042/bj1650395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972 Aug;48(2):422–427. doi: 10.1016/0003-2697(72)90094-2. [DOI] [PubMed] [Google Scholar]
  4. Higgins I. J., Hammond R. C., Sariaslani F. S., Best D., Davies M. M., Tryhorn S. E., Taylor F. Biotransformation of hydrocarbons and related compounds by whole organism suspensions of methane-grown methylosinus trichosporium OB 3b. Biochem Biophys Res Commun. 1979 Jul 27;89(2):671–677. doi: 10.1016/0006-291x(79)90682-x. [DOI] [PubMed] [Google Scholar]
  5. Hou C. T., Patel R., Laskin A. I., Barnabe N., Barist I. Epoxidation of short-chain alkenes by resting-cell suspensions of propane-grown bacteria. Appl Environ Microbiol. 1983 Jul;46(1):171–177. doi: 10.1128/aem.46.1.171-177.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hou C. T., Patel R., Laskin A. I., Barnabe N., Marczak I. Microbial oxidation of gaseous hydrocarbons: production of methyl ketones from their corresponding secondary alcohols by methane- and methanol-grown microbes. Appl Environ Microbiol. 1979 Jul;38(1):135–142. doi: 10.1128/aem.38.1.135-142.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hou C. T., Patel R., Laskin A. I., Barnabe N., Marczak I. Substrate specificity and stereospecificity of nicotinamide adenine dinucleotide-linked alcohol dehydrogenases from methanol-grown yeasts. Appl Environ Microbiol. 1981 Mar;41(3):829–832. doi: 10.1128/aem.41.3.829-832.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hou C. T., Patel R., Laskin A. I., Barnabe N. Microbial oxidation of gaseous hydrocarbons: epoxidation of C2 to C4 n-alkenes by methylotrophic bacteria. Appl Environ Microbiol. 1979 Jul;38(1):127–134. doi: 10.1128/aem.38.1.127-134.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. May S. W., Abbott B. J. Enzymatic epoxidation. I. Alkene epoxidation by the -hydroxylation system of Pseudomonas oleovorans. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1230–1234. doi: 10.1016/0006-291x(72)90842-x. [DOI] [PubMed] [Google Scholar]
  10. May S. W., Abbott B. J. Enzymatic epoxidation. II. Comparison between the epoxidation and hydroxylation reactions catalyzed by the -hydroxylation system of Pseudomonas oleovorans. J Biol Chem. 1973 Mar 10;248(5):1725–1730. [PubMed] [Google Scholar]
  11. Patel R. N., Hou C. T., Laskin A. I., Derelanko P., Felix A. Microbial production of methyl ketones. Purification and properties of a secondary alcohol dehydrogenase from yeast. Eur J Biochem. 1979 Nov;101(2):401–406. doi: 10.1111/j.1432-1033.1979.tb19732.x. [DOI] [PubMed] [Google Scholar]
  12. Patel R. N., Hou C. T., Laskin A. I., Derelanko P., Felix A. Oxidation of secondary alcohols to methyl ketones by yeasts. Appl Environ Microbiol. 1979 Aug;38(2):219–223. doi: 10.1128/aem.38.2.219-223.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Patel R. N., Hou C. T., Laskin A. I., Felix A. Microbial Oxidation of Hydrocarbons: Properties of a Soluble Methane Monooxygenase from a Facultative Methane-Utilizing Organism, Methylobacterium sp. Strain CRL-26. Appl Environ Microbiol. 1982 Nov;44(5):1130–1137. doi: 10.1128/aem.44.5.1130-1137.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stirling D. I., Colby J., Dalton H. A comparison of the substrate and electron-donor specificities of the methane mono-oxygenases from three strains of methane-oxidizing bacteria. Biochem J. 1979 Jan 1;177(1):361–364. doi: 10.1042/bj1770361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. VAN DER LINDEN A. C. EPOXIDATION OF ALPHA-OLEFINS BY HEPTANE-GROWN PSEUDOMONAS CELLS. Biochim Biophys Acta. 1963 Sep 3;77:157–159. doi: 10.1016/0006-3002(63)90484-0. [DOI] [PubMed] [Google Scholar]

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