Abstract
Regenerating protoplasts were obtained from mycelial culture of the mushroom Volvariella volvacea by the action of the lytic enzyme Novozym 234 in the presence of 0.01 M phosphate buffer (pH 6.0) containing 0.6 M NaCl. Regeneration was found to be poor in liquid medium, but more than 50% regeneration was achieved on solid 2% agar medium overlaid with 0.5% agar. Protoplasts of V. volvacea were found to be highly sensitive to the killing action of both UV irradiation and N-methyl-N′-nitro-N-nitrosoguanidine. However, no morphological or auxotrophic mutants could be obtained from protoplasts by chemical mutagenesis. Four types of morphological mutants and one auxotrophic (adenine-negative) mutant were obtained from UV-irradiated protoplasts. The adenine-negative mutant of V. volvacea was found to be stable, not losing auxotrophy on repeated subculture.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ghosh A. K., Banerjee P. C., Sengupta S. Purification and properties of xylan hydrolase from mushroom Termitomyces clypeatus. Biochim Biophys Acta. 1980 Mar 14;612(1):143–152. doi: 10.1016/0005-2744(80)90287-9. [DOI] [PubMed] [Google Scholar]
- Kanda T., Nakakubo S., Wakabayashi K., Nisizawa K. Purification and properties of an exo-cellulase of Avicelase type from a wood-rotting fungus, Irpex lacteus (Polyporus tulipiferae). J Biochem. 1978 Nov;84(5):1217–1226. doi: 10.1093/oxfordjournals.jbchem.a132239. [DOI] [PubMed] [Google Scholar]
- Keller U. Highly Efficient Mutagenesis of Claviceps purpurea by Using Protoplasts. Appl Environ Microbiol. 1983 Sep;46(3):580–584. doi: 10.1128/aem.46.3.580-584.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]