Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Dec;52(6):1419–1421. doi: 10.1128/aem.52.6.1419-1421.1986

Changes in Protein Composition of Three Bacterial Isolates from Marine Waters during Short Periods of Energy and Nutrient Deprivation

Åsa Jouper Jaan 1, Björn Dahllöf 1, Staffan Kjelleberg 1,*
PMCID: PMC239246  PMID: 16347247

Abstract

Two-dimensional gel electrophoresis analysis of and total cell protein determination for three bacterial isolates from marine waters at the onset and after 24 h of energy and nutrient deprivation demonstrated that the three isolates exhibited different pathways of starvation survival. Two strains appeared to synthesize new proteins during starvation.

Full text

PDF
1419

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amy P. S., Morita R. Y. Protein Patterns of Growing and Starved Cells of a Marine Vibrio sp. Appl Environ Microbiol. 1983 Jun;45(6):1748–1752. doi: 10.1128/aem.45.6.1748-1752.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amy P. S., Morita R. Y. Starvation-survival patterns of sixteen freshly isolated open-ocean bacteria. Appl Environ Microbiol. 1983 Mar;45(3):1109–1115. doi: 10.1128/aem.45.3.1109-1115.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kjelleberg S., Hermansson M. Starvation-induced effects on bacterial surface characteristics. Appl Environ Microbiol. 1984 Sep;48(3):497–503. doi: 10.1128/aem.48.3.497-503.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kjelleberg S., Humphrey B. A., Marshall K. C. Effect of interfaces on small, starved marine bacteria. Appl Environ Microbiol. 1982 May;43(5):1166–1172. doi: 10.1128/aem.43.5.1166-1172.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kjelleberg S., Humphrey B. A., Marshall K. C. Initial phases of starvation and activity of bacteria at surfaces. Appl Environ Microbiol. 1983 Nov;46(5):978–984. doi: 10.1128/aem.46.5.978-984.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  8. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  9. Reeve C. A., Amy P. S., Matin A. Role of protein synthesis in the survival of carbon-starved Escherichia coli K-12. J Bacteriol. 1984 Dec;160(3):1041–1046. doi: 10.1128/jb.160.3.1041-1046.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Reeve C. A., Bockman A. T., Matin A. Role of protein degradation in the survival of carbon-starved Escherichia coli and Salmonella typhimurium. J Bacteriol. 1984 Mar;157(3):758–763. doi: 10.1128/jb.157.3.758-763.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Whitaker J. R., Granum P. E. An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Anal Biochem. 1980 Nov 15;109(1):156–159. doi: 10.1016/0003-2697(80)90024-x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES