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. 1983 Jul;46(1):13–16. doi: 10.1128/aem.46.1.13-16.1983

Improved Colorimetric Determination of Cell Wall Chitin in Wood Decay Fungi

George C Chen 1, Bruce R Johnson 1
PMCID: PMC239259  PMID: 16346335

Abstract

The Svennerholm modification of the Elson-Morgan method for glucosamine analysis was evaluated for its applicability to the rapid determination of chitin in wood decay fungi. The evaluation included extent of chromogen interference, sensitivity, color stability, and hydrolysis conditions for maximum release of glucosamine from fungal cell walls. With our further modification, the Svennerholm method was shown to be suitable for rapid quantitative determination of fungal chitin without chromatographic separation of hydrolysate chromogens.

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Selected References

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  1. BLUMENTHAL H. J., ROSEMAN S. Quantitative estimation of chitin in fungi. J Bacteriol. 1957 Aug;74(2):222–224. doi: 10.1128/jb.74.2.222-224.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benson R. L. Assay for 2-amino-2-deoxy-D-glucose in the presence of other primary amines. Carbohydr Res. 1975 Jun;42(1):192–196. doi: 10.1016/s0008-6215(00)84116-6. [DOI] [PubMed] [Google Scholar]
  3. DISCHE Z., BORENFREUND E. A spectrophotometric method for the microdetermination of hexosamines. J Biol Chem. 1950 Jun;184(2):517–522. [PubMed] [Google Scholar]
  4. EXLEY D. The determination of 10-100 wmg. quantities of hexosamine. Biochem J. 1957 Sep;67(1):52–60. doi: 10.1042/bj0670052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Enghofer E., Kress H. An evaluation of the Morgan--Elson assay for 2-amino-2-deoxy sugars. Carbohydr Res. 1979 Nov;76:233–238. doi: 10.1016/0008-6215(79)80022-1. [DOI] [PubMed] [Google Scholar]
  7. GOOD T. A., BESSMAN S. P. DETERMINATION OF GLUCOSAMINE AND GALACTOSAMINE USING BORATE BUFFERS FOR MODIFICATION OF THE ELSON-MORGAN AND MORGAN-ELSON REACTIONS. Anal Biochem. 1964 Nov;9:253–262. doi: 10.1016/0003-2697(64)90183-6. [DOI] [PubMed] [Google Scholar]
  8. Mitchell A. D., Taylor I. E. Cell-wall proteins of Aspergillus niger and Chaetomium globosum. J Gen Microbiol. 1969 Nov;59(1):103–109. doi: 10.1099/00221287-59-1-103. [DOI] [PubMed] [Google Scholar]
  9. Morgan W. T., Elson L. A. A colorimetric method for the determination of N-acetylglucosamine and N-acetylchrondrosamine. Biochem J. 1934;28(3):988–995. doi: 10.1042/bj0280988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REISSIG J. L., STORMINGER J. L., LELOIR L. F. A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem. 1955 Dec;217(2):959–966. [PubMed] [Google Scholar]
  11. SVENNERHOLM L. The determination of hexosamines with special reference to nervous tissue. Acta Soc Med Ups. 1957 Feb 28;61(5-6):287–306. [PubMed] [Google Scholar]
  12. Sietsma J. H., Wessels J. G. Chemical analysis of the hyphal wall of Schizophyllum commune. Biochim Biophys Acta. 1977 Jan 24;496(1):225–239. doi: 10.1016/0304-4165(77)90131-3. [DOI] [PubMed] [Google Scholar]

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