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. 1990;68(1):39–43.

Diagnostic performance indices for immunofluorescent tests and enzyme immunoassays of leishmaniasis sera from northern and north-eastern Brazil.

M C Guimarães 1, B J Celeste 1, E L Franco 1
PMCID: PMC2393007  PMID: 2189584

Abstract

A total of 341 sera were screened for anti-Leishmania IgA, IgG, and IgM antibodies by immunofluorescent (IF) tests and enzyme immunoassay (ELISA). Altogether, 292 of the sera originated from patients with clinically as well as parasitologically diagnosed (positive lesion imprint or the Montenegro skin test) cutaneous leishmaniasis; 49 of the sera were from controls from the same base population. In terms of diagnostic performance, the ELISAs for IgG and IgM yielded indices of diagnostic utility, and the positive predictive value for the IgG-ELISA was 94.6%. A remarkably high specificity (100%) was obtained with the IgA-IF test, but its sensitivity was very low.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anthony R. L., Christensen H. A., Johnson C. M. Micro enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of New World leishmaniasis. Am J Trop Med Hyg. 1980 Mar;29(2):190–194. doi: 10.4269/ajtmh.1980.29.190. [DOI] [PubMed] [Google Scholar]
  2. Anthony R. L., Williams K. M., Sacci J. B., Rubin D. C. Subcellular and taxonomic specificity of monoclonal antibodies to New World Leishmania. Am J Trop Med Hyg. 1985 Nov;34(6):1085–1094. doi: 10.4269/ajtmh.1985.34.1085. [DOI] [PubMed] [Google Scholar]
  3. Camargo M. E., Leser P. G., Rocca A. Rheumatoid factors as a cause for false positive IgM anti-toxoplasma fluorescent tests. A technique for specific results. Rev Inst Med Trop Sao Paulo. 1972 Sep-Oct;14(5):310–313. [PubMed] [Google Scholar]
  4. Cuba Cuba C. A., Marsden P. D., Barreto A. C., Rocha R., Sampaio R. R., Patzlaff L. Parasitologic and immunologic diagnosis of American (mucocutaneous) leishmaniasis. Bull Pan Am Health Organ. 1981;15(3):249–259. [PubMed] [Google Scholar]
  5. Miles M. A. Biochemical identification of the leishmanias. Bull Pan Am Health Organ. 1985;19(4):343–353. [PubMed] [Google Scholar]
  6. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  7. Shaw J. J., Lainson R. Leishmaniasis in Brazil. XIV. Leishmanial and trypanosomal IgA antibody in patients with leishmaniasis and Chagas's disease. Trans R Soc Trop Med Hyg. 1981;75(2):254–257. doi: 10.1016/0035-9203(81)90329-1. [DOI] [PubMed] [Google Scholar]

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