Skip to main content
NCBI home page
Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1990;68(Suppl):145–151.

Delayed-type hypersensitivity and protection in mice following immunization with Plasmodium berghei sporozoites.

J P Verhave 1, G T Strickland 1
PMCID: PMC2393028  PMID: 2094579

Abstract

The measurement of footpad swelling (FPS) following the inoculation of sporozoite antigen (Ag) into the hind footpad (HFP) of outbred mice was used as an in vivo test of delayed-type hypersensitivity (DTH) to attenuated sporozoite immunization. An attempt was made to correlate DTH with protective antisporozoite immunity. The optimum time for testing DTH following a single intravenous immunization was four days. The optimum sensitizing dose was 1 x 10(5) attenuated sporozoites. A single immunization gave a greater FPS than multiple immunizations. Mice immunized with irradiation-attenuated sporozoites could be protected from a lethal challenge with nonattenuated sporozoites. The FPS response to mosquito debris Ag was minimal in mice sensitized by bites from infected mosquitos. Nude mice failed to develop an FPS response and could not mount a protective immunity with attenuated sporozoites. However, splenectomized mice did have an FPS response following sensitization. Mice infected with blood-stage parasites had negligible FPS response to sporozoite Ag. The FPS response to sporozoite Ag proved to be a correlate of DTH in mice sensitized with sporozoites.

Full text

PDF
145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Finerty J. F., Krehl E. P. Delayed immune reactions in mice immunized with malarial antigen. Am J Trop Med Hyg. 1977 May;26(3):377–381. doi: 10.4269/ajtmh.1977.26.377. [DOI] [PubMed] [Google Scholar]
  2. Golenser J., Heeren J., Verhave J. P., Kaay H. J., Meuwissen J. H. Crossreactivity with sporozoites, exoerythrocytic forms and blood schizonts of Plasmodium berghei in indirect fluorescent antibody tests with sera of rats immunized with sporozoites or infected blood. Clin Exp Immunol. 1977 Jul;29(1):43–51. [PMC free article] [PubMed] [Google Scholar]
  3. Lagrange P. H., Mackaness G. B. A stable form of delayed-type hypersensitivity. J Exp Med. 1975 Jan 1;141(1):82–96. doi: 10.1084/jem.141.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lagrange P. H., Mackaness G. B., Miller T. E. Influence of dose and route of antigen injection on the immunological induction of T cells. J Exp Med. 1974 Mar 1;139(3):528–542. doi: 10.1084/jem.139.3.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mackaness G. B., Lagrange P. H., Miller T. E., Ishibashi T. Feedback inhibition of specifically sensitized lymphocytes. J Exp Med. 1974 Mar 1;139(3):543–559. doi: 10.1084/jem.139.3.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nardin E. H., Nussenzweig R. S. Stage-specific antigens on the surface membrane of sporozoites of malaria parasites. Nature. 1978 Jul 6;274(5666):55–57. doi: 10.1038/274055a0. [DOI] [PubMed] [Google Scholar]
  7. Pacheco N. D., Strome C. P., Mitchell F., Bawden M. P., Beaudoin R. L. Rapid, large-scale isolation of Plasmodium berghei sporozoites from infected mosquitoes. J Parasitol. 1979 Jun;65(3):414–417. [PubMed] [Google Scholar]
  8. Sadoff J. C., Ballou W. R., Baron L. S., Majarian W. R., Brey R. N., Hockmeyer W. T., Young J. F., Cryz S. J., Ou J., Lowell G. H. Oral Salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria. Science. 1988 Apr 15;240(4850):336–338. doi: 10.1126/science.3281260. [DOI] [PubMed] [Google Scholar]
  9. Schofield L., Villaquiran J., Ferreira A., Schellekens H., Nussenzweig R., Nussenzweig V. Gamma interferon, CD8+ T cells and antibodies required for immunity to malaria sporozoites. Nature. 1987 Dec 17;330(6149):664–666. doi: 10.1038/330664a0. [DOI] [PubMed] [Google Scholar]
  10. Spitalny G. L., Nussenzweig R. S. Plasmodium berghei: relationship between protective immunity and anti-sporozoite (CSP) antibody in mice. Exp Parasitol. 1973 Feb;33(1):168–178. doi: 10.1016/0014-4894(73)90022-2. [DOI] [PubMed] [Google Scholar]
  11. Spitalny G. L., Rivera-Ortiz C. I., Nussenzweig R. S. Plasmodium berghei: the spleen in sporozoite-induced immunity to mouse malaria. Exp Parasitol. 1976 Oct;40(2):179–188. doi: 10.1016/0014-4894(76)90080-1. [DOI] [PubMed] [Google Scholar]
  12. Spitalny G. L., Verhave J. P., Meuwissen J. H., Nussenzweig R. S. Plasmodium berghei: T cell dependence of sporozoite-induced immunity in rodents. Exp Parasitol. 1977 Jun;42(1):73–81. doi: 10.1016/0014-4894(77)90063-7. [DOI] [PubMed] [Google Scholar]
  13. Vanderberg J. P. Development of infectivity by the Plasmodium berghei sporozoite. J Parasitol. 1975 Feb;61(1):43–50. [PubMed] [Google Scholar]
  14. Verhave J. P., Strickland G. T., Jaffe H. A., Ahmed A. Studies on the transfer of protective immunity with lymphoid cells from mice immune to malaria sporozoites. J Immunol. 1978 Sep;121(3):1031–1033. [PubMed] [Google Scholar]
  15. Weidanz W. P., Long C. A. The role of T cells in immunity to malaria. Prog Allergy. 1988;41:215–252. [PubMed] [Google Scholar]
  16. Weiss W. R., Sedegah M., Beaudoin R. L., Miller L. H., Good M. F. CD8+ T cells (cytotoxic/suppressors) are required for protection in mice immunized with malaria sporozoites. Proc Natl Acad Sci U S A. 1988 Jan;85(2):573–576. doi: 10.1073/pnas.85.2.573. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Bulletin of the World Health Organization are provided here courtesy of World Health Organization

RESOURCES