Abstract
The restricted access to Plasmodium falciparum liver stages has greatly limited the analysis of the antigenic content of that stage. Due to the lack of material to perform immunochemical studies, of access to mRNA, and of monoclonal probes, we decided to screen a genomic library with stage-restricted human antibodies. This strategy led to the identification of a large number of DNA fragments encoding both sporozoite specific as well as liver-stage specific epitopes. Following the initial characterization of one liver-stage antigen, further screening was performed by using additional selective human antibodies. These were defined as having a high degree of reactivity with native antigens on either of the two stages while being negative with the already known molecules of the two stages. From this second screening and the study of cross-reactions, several subsets of DNA clones expressing antigens present on the surface of sporozoites, or in liver stages, or in both, could be identified. In exposed individuals a high prevalence of antibodies to several of these antigens was found.
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- Druilhe P., Puebla R. M., Miltgen F., Perrin L., Gentilini M. Species- and stage-specific antigens in exoerythrocytic stages of Plasmodium falciparum. Am J Trop Med Hyg. 1984 May;33(3):336–341. doi: 10.4269/ajtmh.1984.33.336. [DOI] [PubMed] [Google Scholar]
- Galey B., Druilhe P., Ploton I., Desgranges C., Asavanich A., Harinasuta T., Marchand C., Brahimi K., Charoenvit Y., Paul C. Evidence for diversity of Plasmodium falciparum sporozoite surface antigens derived from analysis of antibodies elicited in humans. Infect Immun. 1990 Sep;58(9):2995–3001. doi: 10.1128/iai.58.9.2995-3001.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerin-Marchand C., Druilhe P., Galey B., Londono A., Patarapotikul J., Beaudoin R. L., Dubeaux C., Tartar A., Mercereau-Puijalon O., Langsley G. A liver-stage-specific antigen of Plasmodium falciparum characterized by gene cloning. Nature. 1987 Sep 10;329(6135):164–167. doi: 10.1038/329164a0. [DOI] [PubMed] [Google Scholar]
- Mazier D., Landau I., Druilhe P., Miltgen F., Guguen-Guillouzo C., Baccam D., Baxter J., Chigot J. P., Gentilini M. Cultivation of the liver forms of Plasmodium vivax in human hepatocytes. 1984 Jan 26-Feb 1Nature. 307(5949):367–369. doi: 10.1038/307367a0. [DOI] [PubMed] [Google Scholar]