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. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983

Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

D G Anderson, L L McKay
PMCID: PMC239314  PMID: 6416164

Abstract

A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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