Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1983 Sep;46(3):636–641. doi: 10.1128/aem.46.3.636-641.1983

Detection and enumeration of virulent Yersinia enterocolitica in food by DNA colony hybridization.

W E Hill, W L Payne, C C Aulisio
PMCID: PMC239328  PMID: 6639017

Abstract

A portion of a 44-megadalton plasmid found in Yersinia enterocolitica 8081 was used as a genetic probe to differentiate virulent and nonvirulent strains of the species. A DNA colony hybridization technique was employed. Three BamHI restriction endonuclease fragments labeled with 32P by nick translation were hybridized to lysed colonies of pure cultures, mixtures of virulent and nonvirulent cells, and portions of a food sample artificially contaminated with virulent Y. enterocolitica. The results of the colony hybridization test for virulence were the same as those obtained by the autoagglutination and suckling mouse tests. DNA colony hybridization detects pathogenic Y. enterocolitica in foods without the need for enrichment or pure cultures.

Full text

PDF
636

Images in this article

638Image
on p.638
638Image
on p.638
639Image
on p.639
640Image
on p.640

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aulisio C. C., Hill W. E., Stanfield J. T., Sellers R. L., Jr Evaluation of virulence factor testing and characteristics of pathogenicity in Yersinia enterocolitica. Infect Immun. 1983 Apr;40(1):330–335. doi: 10.1128/iai.40.1.330-335.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ben-Gurion R., Shafferman A. Essential virulence determinants of different Yersinia species are carried on a common plasmid. Plasmid. 1981 Mar;5(2):183–187. doi: 10.1016/0147-619x(81)90019-6. [DOI] [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Black R. E., Jackson R. J., Tsai T., Medvesky M., Shayegani M., Feeley J. C., MacLeod K. I., Wakelee A. M. Epidemic Yersinia enterocolitica infection due to contaminated chocolate milk. N Engl J Med. 1978 Jan 12;298(2):76–79. doi: 10.1056/NEJM197801122980204. [DOI] [PubMed] [Google Scholar]
  5. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gemski P., Lazere J. R., Casey T., Wohlhieter J. A. Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infect Immun. 1980 Jun;28(3):1044–1047. doi: 10.1128/iai.28.3.1044-1047.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harvey S., Greenwood J. R., Pickett M. J., Mah R. A. Recovery of Yersinia enterocolitica from streams and lakes of California. Appl Environ Microbiol. 1976 Sep;32(3):352–354. doi: 10.1128/aem.32.3.352-354.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hill W. E., Carlisle C. L. Loss of plasmids during enrichment for Escherichia coli. Appl Environ Microbiol. 1981 Apr;41(4):1046–1048. doi: 10.1128/aem.41.4.1046-1048.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill W. E., Madden J. M., McCardell B. A., Shah D. B., Jagow J. A., Payne W. L., Boutin B. K. Foodborne enterotoxigenic Escherichia coli: detection and enumeration by DNA colony hybridization. Appl Environ Microbiol. 1983 Apr;45(4):1324–1330. doi: 10.1128/aem.45.4.1324-1330.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hughes D. Isolation of Yersinia enterocolitica from milk and a dairy farm in Australia. J Appl Bacteriol. 1979 Feb;46(1):125–130. doi: 10.1111/j.1365-2672.1979.tb02589.x. [DOI] [PubMed] [Google Scholar]
  11. Kay B. A., Wachsmuth K., Gemski P. New virulence-associated plasmid in Yersinia enterocolitica. J Clin Microbiol. 1982 Jun;15(6):1161–1163. doi: 10.1128/jcm.15.6.1161-1163.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laird W. J., Cavanaugh D. C. Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980 Apr;11(4):430–432. doi: 10.1128/jcm.11.4.430-432.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
  14. Lee W. H. Testing for the recovery of Yersinia enterocolitica in foods and their ability to invade HeLa cells. Contrib Microbiol Immunol. 1979;5:228–233. [PubMed] [Google Scholar]
  15. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pai C. H., Mors V. Production of enterotoxin by Yersinia enterocolitica. Infect Immun. 1978 Mar;19(3):908–911. doi: 10.1128/iai.19.3.908-911.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Portnoy D. A., Falkow S. Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis. J Bacteriol. 1981 Dec;148(3):877–883. doi: 10.1128/jb.148.3.877-883.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schiemann D. A., Devenish J. A. Virulence of Yersinia enterocolitica determined by lethality in Mongolian gerbils and by the Serény test. Infect Immun. 1980 Aug;29(2):500–506. doi: 10.1128/iai.29.2.500-506.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schiemann D. A., Toma S. Isolation of Yersinia enterocolitica from raw milk. Appl Environ Microbiol. 1978 Jan;35(1):54–58. doi: 10.1128/aem.35.1.54-58.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zink D. L., Feeley J. C., Wells J. G., Vanderzant C., Vickery J. C., Roof W. D., O'Donovan G. A. Plasmid-mediated tissue invasiveness in Yersinia enterocolitica. Nature. 1980 Jan 10;283(5743):224–226. doi: 10.1038/283224a0. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES