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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Biomaterials. 2008 Apr 2;29(17):2680–2688. doi: 10.1016/j.biomaterials.2008.03.009

Figure 7. Effect of Bcl-2 levels on transfection activity of luciferase siRNA complexes.

Figure 7

Cells were co-transfected for 4 h in the absence of FBS with 0.5 μg Luc-pDNA and 0.2 μg siRNA targeted against luciferase, complexed with PLL/rPLL (N:P 2) to which DOTAP was added to form ternary complexes. Luciferase expression was measured after 24 h. Knockdown of luciferase expression was calculated by comparison with cells transfected with pDNA only. Redox/Non-redox is the ratio (± SD, n=3) of knockdown activity of rPLL-to-PLL complexes. Secondary axis (●) shows % knockdown mediated by rPLL complexes.