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. 1983 Aug;46(2):511–514. doi: 10.1128/aem.46.2.511-514.1983

Isolation and Characterization of a Xylanase from Bacillus subtilis

Roger Bernier Jr 1, Michel Desrochers 1, Lubomir Jurasek 1, Michael G Paice 1
PMCID: PMC239434  PMID: 16346375

Abstract

Partial characterization of an extracellular xylanase isolated by chromatography from Bacillus subtilis gave a molecular weight of 32,000 and optimum pH and temperature of 5.0 and 50°C, respectively. Km and Vmax values, determined with a soluble larchwood xylan, were 0.16% and 7.0 × 103 μmol min−1 mg−1 of enzyme respectively. The amino acid composition showed more basic amino acid residues than in a previously characterized xylanase from a white-rot fungus.

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Selected References

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