Sir,
The characterisation of the TIMP-1 gene was published in Wang et al (1998). The TIMP-1 gene was cloned from PC-3 ML genomic DNA. The Pst1–Xba1 fragment at the 5′end of the Pst1 4.0-kilobase TIMP-1 genomic clone was subcloned into plasmid PUC 19. This fragment was sequenced from both ends on opposite strands with the Sequenase Virgin 2.0 sequencing system (US Biochemical). Each strand was sequenced with both the Klenow fragment and reverse transcriptase by standard dideoxy procedures. Blast analysis of the 5′ promoter region revealed a 97% homology with Homo sapiens TIMP-1 promoter region (chromosome X genomic contig, NT 011568). Blast analysis of the coding region revealed a 97% homology with human TIMP-1 (Docherty et al, 1985) and 96% homology with the coding region of the E. coli TIMP-1.
References
- Docherty AJ, Lyons A, Smith BJ, Wright EM, Stephens PE, Harris TJ, Murphy G, Reynolds JJ (1985) Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity. Nature 318: 666–669 [DOI] [PubMed] [Google Scholar]
- Wang M, Hu Y, Shima I, Stearns ME (1998) Identification of positive and negative regulator elements for the tissue inhibitor of metalloproteinase 1 gene. Oncol Res 10: 219–233 [PubMed] [Google Scholar]