Abstract
A simple and rapid method was developed for the extraction of proteins from both pathogenic and nonpathogenic bacteria. The method involves the treatment of cells with acetone followed by sodium dodecyl sulfate extraction of cellular proteins. Polyacrylamide gel electrophoresis revealed that the protein composition of extracts made by this method was comparable to that of extracts made by established methods, namely, sonication and agitation with beads. This technique has been successfully applied to the extraction of proteins from a wide variety of bacteria, including pathogens.
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- Ames G. F. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions. J Biol Chem. 1974 Jan 25;249(2):634–644. [PubMed] [Google Scholar]
- BOWERS L. E., WILLIAMS O. B. EFFECT OF ARGININE ON GROWTH AND LYSIS OF CLOSTRIDIUM BOTULINUM. J Bacteriol. 1963 May;85:1175–1176. doi: 10.1128/jb.85.5.1175-1176.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Basch J. J., Farrell H. M., Jr Charge separation of proteins complexed with sodium dodecyl sulfate by acid gel electrophoresis in the presence of cetyltrimethylammonium bromide. Biochim Biophys Acta. 1979 Mar 27;577(1):125–131. doi: 10.1016/0005-2795(79)90014-x. [DOI] [PubMed] [Google Scholar]
- Bhaduri S., Raskas H. J., Green M. Procedure for the preparation of milligram quantities of adenovirus messenger ribonucleic acid. J Virol. 1972 Dec;10(6):1126–1129. doi: 10.1128/jvi.10.6.1126-1129.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ezaki T., Suzuki S. Achromopeptidase for lysis of anaerobic gram-positive cocci. J Clin Microbiol. 1982 Nov;16(5):844–846. doi: 10.1128/jcm.16.5.844-846.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Thompson J., Chassy B. M. Uptake and metabolism of sucrose by Streptococcus lactis. J Bacteriol. 1981 Aug;147(2):543–551. doi: 10.1128/jb.147.2.543-551.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]