Abstract
Campylobacter jejuni has been incriminated in several large waterborne outbreaks, but it has rarely been isolated from water itself. Better methodology is needed for the isolation of C. jejuni from water. We evaluated three types of 0.45-micron microporous filters and three different pore sizes of positively charged depth filters for their ability to recover C. jejuni from seeded, sterile tap and surface water. The microporous filters tested were Millipore HA, Gelman GN6, and Zetapor. Three pore sizes of Zeta Plus depth filters (05S, 30S, and 50S) were evaluated by using an adsorption-elution technique. The overall percent recovery in both tap and surface water by microporous filters was: Zetapor, 66%; Millipore HA, 33%; and Gelman GN6, 33%. Adsorption-elution with Zeta Plus 50S allowed 89% recovery of C. jejuni. These data suggest that both the positively charged Zetapor microporous filter and the Zeta Plus 50S depth filter are effective filters for the recovery of C. jejuni from water.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Blaser M. J., Berkowitz I. D., LaForce F. M., Cravens J., Reller L. B., Wang W. L. Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med. 1979 Aug;91(2):179–185. doi: 10.7326/0003-4819-91-2-179. [DOI] [PubMed] [Google Scholar]
- Blaser M. J., Hardesty H. L., Powers B., Wang W. L. Survival of Campylobacter fetus subsp. jejuni in biological milieus. J Clin Microbiol. 1980 Apr;11(4):309–313. doi: 10.1128/jcm.11.4.309-313.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blaser M. J., Reller L. B. Campylobacter enteritis. N Engl J Med. 1981 Dec 10;305(24):1444–1452. doi: 10.1056/NEJM198112103052404. [DOI] [PubMed] [Google Scholar]
- Pai C. H., Sorger S., Lackman L., Sinai R. E., Marks M. I. Campylobacter gastroenteritis in children. J Pediatr. 1979 Apr;94(4):589–591. doi: 10.1016/s0022-3476(79)80016-5. [DOI] [PubMed] [Google Scholar]
- Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogt R. L., Sours H. E., Barrett T., Feldman R. A., Dickinson R. J., Witherell L. Campylobacter enteritis associated with contaminated water. Ann Intern Med. 1982 Mar;96(3):292–296. doi: 10.7326/0003-4819-96-3-292. [DOI] [PubMed] [Google Scholar]