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. 1983 Nov;46(5):1080–1083. doi: 10.1128/aem.46.5.1080-1083.1983

Confirmation of Occurrence of Hydroxamate Siderophores in Soil by a Novel Escherichia coli Bioassay

Paul E Powell 1,†, Paul J Szaniszlo 1, C P Patrick Reid 1
PMCID: PMC239522  PMID: 16346415

Abstract

The occurrence of ferrichrome-type hydroxamate siderophores in soil was confirmed. In the presence of the iron-scavenging chelator ethylenediamine[di(o-hydroxyphenylacetic)acid], soil extract stimulated the growth of an Escherichia coli strain possessing the ferrichrome transport protein (TonA) but did not stimulate growth of a strain lacking this protein (TonA−). The siderophore concentration in a 1:1 (soil-water) extract was estimated to be approximately 78 nM. Specificity of the assay was supported by the absence of significant differential strain responses to ferric citrate, ferric 2,3-dihydroxybenzoate, enterochelin, ferrioxamine B, coprogen, and triacetylfusigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coulton J. W., Naegeli H. U., Braun V. Iron supply of Escherichia coli with polymer-bound ferricrocin. Eur J Biochem. 1979 Aug 15;99(1):39–47. doi: 10.1111/j.1432-1033.1979.tb13228.x. [DOI] [PubMed] [Google Scholar]
  2. Emery T. Role of ferrichrome as a ferric ionophore in Ustilago sphaerogena. Biochemistry. 1971 Apr 13;10(8):1483–1488. doi: 10.1021/bi00784a033. [DOI] [PubMed] [Google Scholar]
  3. Frost G. E., Rosenberg H. Relationship between the tonB locus and iron transport in Escherichia coli. J Bacteriol. 1975 Nov;124(2):704–712. doi: 10.1128/jb.124.2.704-712.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hantke K., Braun V. Membrane receptor dependent iron transport in Escherichia coli. FEBS Lett. 1975 Jan 1;49(3):301–305. doi: 10.1016/0014-5793(75)80771-x. [DOI] [PubMed] [Google Scholar]
  5. Hartmann A., Braun V. Iron transport in Escherichia coli: uptake and modification of ferrichrome. J Bacteriol. 1980 Jul;143(1):246–255. doi: 10.1128/jb.143.1.246-255.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kadner R. J., Heller K., Coulton J. W., Braun V. Genetic control of hydroxamate-mediated iron uptake in Escherichia coli. J Bacteriol. 1980 Jul;143(1):256–264. doi: 10.1128/jb.143.1.256-264.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Leong J., Neilands J. B. Mechanisms of siderophore iron transport in enteric bacteria. J Bacteriol. 1976 May;126(2):823–830. doi: 10.1128/jb.126.2.823-830.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miles A. A., Khimji P. L. Enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity. J Med Microbiol. 1975 Nov;8(4):477–490. doi: 10.1099/00222615-8-4-477. [DOI] [PubMed] [Google Scholar]
  9. Moore R. E., Emery T. Nalpha-acetylfusarinines: isolation, characterization, and properties. Biochemistry. 1976 Jun 29;15(13):2719–2723. doi: 10.1021/bi00658a001. [DOI] [PubMed] [Google Scholar]
  10. Neilands J. B. Iron absorption and transport in microorganisms. Annu Rev Nutr. 1981;1:27–46. doi: 10.1146/annurev.nu.01.070181.000331. [DOI] [PubMed] [Google Scholar]
  11. Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
  12. Stutz E. Aufnahme von Ferrioxamin B durch Tomatenpflanzen. Experientia. 1964 Aug 15;20(8):430–431. doi: 10.1007/BF02152129. [DOI] [PubMed] [Google Scholar]
  13. Warner P. J., Williams P. H., Bindereif A., Neilands J. B. ColV plasmid-specific aerobactin synthesis by invasive strains of Escherichia coli. Infect Immun. 1981 Aug;33(2):540–545. doi: 10.1128/iai.33.2.540-545.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wayne R., Neilands J. B. Evidence for common binding sites for ferrichrome compounds and bacteriophage phi 80 in the cell envelope of Escherichia coli. J Bacteriol. 1975 Feb;121(2):497–503. doi: 10.1128/jb.121.2.497-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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