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. 1983 Nov;46(5):1176–1181. doi: 10.1128/aem.46.5.1176-1181.1983

Metabolism of Halophenols by 2,4,5-trichlorophenoxyacetic acid-degrading Pseudomonas cepacia.

J S Karns, J J Kilbane, S Duttagupta, A M Chakrabarty
PMCID: PMC239537  PMID: 6651297

Abstract

Resting cells of 2,4,5-trichlorophenoxyacetic acid-grown Pseudomonas cepacia AC1100 were able to completely and rapidly dechlorinate several chlorine-substituted phenols, including 2,4,5-trichlorophenol, 2,3,4,6-tetrachlorophenol, and pentachlorophenol. Several other trichlorophenols were only partially dechlorinated. The evidence suggests that 2,4,5-trichlorophenol is an intermediate in the degradation of 2,4,5-trichlorophenoxyacetic acid by strain AC1100. Moreover, although strain AC1100 was isolated by selection for growth on a chlorinated aromatic compound, brominated and fluorinated analogs were efficiently dehalogenated by strain AC1100 resting cells, whereas an iodinated analog was poorly dehalogenated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bevenue A., Beckman H. Pentachlorophenol: a discussion of its properties and its occurrence as a residue in human and animal tissues. Residue Rev. 1967;19:83–134. doi: 10.1007/978-1-4615-8425-4_5. [DOI] [PubMed] [Google Scholar]
  2. Dorn E., Hellwig M., Reineke W., Knackmuss H. J. Isolation and characterization of a 3-chlorobenzoate degrading pseudomonad. Arch Microbiol. 1974;99(1):61–70. doi: 10.1007/BF00696222. [DOI] [PubMed] [Google Scholar]
  3. Evans W. C., Smith B. S., Fernley H. N., Davies J. I. Bacterial metabolism of 2,4-dichlorophenoxyacetate. Biochem J. 1971 May;122(4):543–551. doi: 10.1042/bj1220543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldman P., Milne G. W., Keister D. B. Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases. J Biol Chem. 1968 Jan 25;243(2):428–434. [PubMed] [Google Scholar]
  5. Hammond A. L. Chemical pollution: polychlorinated biphenyls. Science. 1972 Jan 14;175(4018):155–156. doi: 10.1126/science.175.4018.155. [DOI] [PubMed] [Google Scholar]
  6. Karns J. S., Duttagupta S., Chakrabarty A. M. Regulation of 2,4,5-trichlorophenoxyacetic acid and chlorophenol metabolism in Pseudomonas cepacia AC1100. Appl Environ Microbiol. 1983 Nov;46(5):1182–1186. doi: 10.1128/aem.46.5.1182-1186.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kellogg S. T., Chatterjee D. K., Chakrabarty A. M. Plasmid-assisted molecular breeding: new technique for enhanced biodegradation of persistent toxic chemicals. Science. 1981 Dec 4;214(4525):1133–1135. doi: 10.1126/science.7302584. [DOI] [PubMed] [Google Scholar]
  8. Kilbane J. J., Chatterjee D. K., Karns J. S., Kellogg S. T., Chakrabarty A. M. Biodegradation of 2,4,5-trichlorophenoxyacetic acid by a pure culture of Pseudomonas cepacia. Appl Environ Microbiol. 1982 Jul;44(1):72–78. doi: 10.1128/aem.44.1.72-78.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Motosugi K., Esaki N., Soda K. Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp. J Bacteriol. 1982 May;150(2):522–527. doi: 10.1128/jb.150.2.522-527.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Omori T., Alexander M. Bacterial dehalogenation of halogenated alkanes and fatty acids. Appl Environ Microbiol. 1978 May;35(5):867–871. doi: 10.1128/aem.35.5.867-871.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reineke W., Knackmuss H. J. Chemical structure and biodegradability of halogenate aromatic compounds. Substituent effects on 1,2-dioxygenation of benzoic acid. Biochim Biophys Acta. 1978 Sep 6;542(3):412–423. doi: 10.1016/0304-4165(78)90372-0. [DOI] [PubMed] [Google Scholar]
  13. Schreiber A., Hellwig M., Dorn E., Reineke W., Knackmuss H. J. Critical Reactions in Fluorobenzoic Acid Degradation by Pseudomonas sp. B13. Appl Environ Microbiol. 1980 Jan;39(1):58–67. doi: 10.1128/aem.39.1.58-67.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stanlake G. J., Finn R. K. Isolation and characterization of a pentachlorophenol-degrading bacterium. Appl Environ Microbiol. 1982 Dec;44(6):1421–1427. doi: 10.1128/aem.44.6.1421-1427.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weinbach E. C. BIOCHEMICAL BASIS FOR THE TOXICITY OF PENTACHLOROPHENOL. Proc Natl Acad Sci U S A. 1957 May 15;43(5):393–397. doi: 10.1073/pnas.43.5.393. [DOI] [PMC free article] [PubMed] [Google Scholar]

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