Abstract
The expression of the degradative genes encoding 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 2,4,5-trichlorophenol (2,4,5-TCP), and pentachlorophenol (PCP) dechlorination in a 2,4,5-T-degrading strain of Pseudomonas cepacia was examined during growth on alternate carbon sources. The dechlorination mechanisms for all three compounds were expressed in 2,4,5-T- and 2,4,5-TCP-grown cells but were not expressed in cells grown on succinate, glucose, or lactate. The addition of 2,4,5-TCP or PCP to cells grown on succinate or lactate resulted in the expression of the 2,4,5-TCP dechlorination mechanism in resting cells after 1-h lag. This expression was prevented by the presence of chloramphenicol in the resting cell suspension. Succinate-plus-PCP-grown resting cells preincubated with 2,4,5-TCP fully induced the trichlorophenol dechlorination system and partially induced the PCP dechlorination system. Preincubation of succinate-plus-PCP-grown resting cells with PCP induced neither the 2,4,5-TCP nor the PCP dechlorinating system. Succinate-grown resting cells converted 2,4,5-T to 2,4,5-TCP even in the presence of chloramphenicol. Thus, the data indicate that the enzyme(s) which converts 2,4,5-T to 2,4,5-TCP is constitutively expressed, whereas those that convert 2,4,5-TCP to central intermediates are induced by 2,4,5-TCP but not by 2,4,5-T or PCP and are repressed in the presence of an alternate carbon source.
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- Hylemon P. B., Phibbs P. V., Jr Independent regulation of hexose catabolizing enzymes and glucose transport activity in Pseudomonas aeruginosa. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1041–1048. doi: 10.1016/0006-291x(72)90813-3. [DOI] [PubMed] [Google Scholar]
- Karns J. S., Kilbane J. J., Duttagupta S., Chakrabarty A. M. Metabolism of Halophenols by 2,4,5-trichlorophenoxyacetic acid-degrading Pseudomonas cepacia. Appl Environ Microbiol. 1983 Nov;46(5):1176–1181. doi: 10.1128/aem.46.5.1176-1181.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kight-Olliff L. C., Fitzgerald J. W. Inhibition of enzyme induction in Pseudomonas aeruginosa by exogenous nucleotides. Can J Microbiol. 1978 Jul;24(7):811–817. doi: 10.1139/m78-136. [DOI] [PubMed] [Google Scholar]
- Kilbane J. J., Chatterjee D. K., Chakrabarty A. M. Detoxification of 2,4,5-trichlorophenoxyacetic acid from contaminated soil by Pseudomonas cepacia. Appl Environ Microbiol. 1983 May;45(5):1697–1700. doi: 10.1128/aem.45.5.1697-1700.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilbane J. J., Chatterjee D. K., Karns J. S., Kellogg S. T., Chakrabarty A. M. Biodegradation of 2,4,5-trichlorophenoxyacetic acid by a pure culture of Pseudomonas cepacia. Appl Environ Microbiol. 1982 Jul;44(1):72–78. doi: 10.1128/aem.44.1.72-78.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Midgley M., Dawes E. A. The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa. Biochem J. 1973 Feb;132(2):141–154. doi: 10.1042/bj1320141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Siegel L. S., Hylemon P. B., Phibbs P. V., Jr Cyclic adenosine 3',5'-monophosphate levels and activities of adenylate cyclase and cyclic adenosine 3',5'-monophosphate phosphodiesterase in Pseudomonas and Bacteroides. J Bacteriol. 1977 Jan;129(1):87–96. doi: 10.1128/jb.129.1.87-96.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiwari N. P., Campbell J. J. Enzymatic control of the metabolic activity of Pseudomonas aeruginosa grown in glucose or succinate media. Biochim Biophys Acta. 1969 Dec 30;192(3):395–401. doi: 10.1016/0304-4165(69)90388-2. [DOI] [PubMed] [Google Scholar]
- Weinbach E. C. BIOCHEMICAL BASIS FOR THE TOXICITY OF PENTACHLOROPHENOL. Proc Natl Acad Sci U S A. 1957 May 15;43(5):393–397. doi: 10.1073/pnas.43.5.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whiting P. H., Midgley M., Dawes E. A. The regulation of transport of glucose, gluconate and 2-oxogluconate and of glucose catabolism in Pseudomonas aeruginosa. Biochem J. 1976 Mar 15;154(3):659–668. doi: 10.1042/bj1540659. [DOI] [PMC free article] [PubMed] [Google Scholar]