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. 1999 Sep;3(2):107–113. doi: 10.1080/13577149977730

Expression of Smooth Muscle Calponin in Synovial Sarcoma

Hidefumi Ono 1, Hideki Yoshikawa 1,✉, Takafumi Ueda 1, Hisako Yamamura 2, Ikuo Kudawara 1, Masayuki Manou 3, Shingo Ishiguro 3, Hiroko Funai 3, Yumiko Koyanagi 3, Nobuhito Araki 1, Nobuyuki Hashimoto 4, Hiroshi Sonobe 5, Masaharu Tatsuta 6, Katsuhito Takahashi 2,7,8
PMCID: PMC2395415  PMID: 18521272

Abstract

Purpose. Histogenesis of synovial sarcoma remains controversial and reliable molecular markers for diagnosis are necessary. Expression of basic calponin, a smooth muscle differentiation-specific actin-binding protein, was studied in synovial sarcoma.

Subjects and Methods. The basic calponin gene and the gene product were analyzed by reverse transcription PCR analysis (RT-PCR) and immunohistochemistry in 14 synovial sarcomas and a human synovial sarcoma cell line (HS-SY-II).

Results and Discussion. Immunoreactivity for basic calponin was detected in the cytoplasm of 6 synovial sarcomas (43% positive). In the basic calponin-positive tumors and the HS-SY-II cells, expression for smooth muscle-specific genes, including basic calponin and SM22α , was detected by RT-PCR, suggesting a lineage relationship between synovial sarcoma cells and smooth muscle-like mesenchymal cells.

Conclusions. A subset of synovial sarcomas expressing the basic calponin gene and the gene product were identified. The basic calponin may have potential utility as a novel molecular marker identifying certain synovial sarcomas.

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