Abstract
In order to characterize parasite-induced host cell membrane antigens, the plasma membranes of Plasmodium knowlesi-infected rhesus erythrocytes have been compared with those of normal red cells and purified schizonts by immunochemical and biochemical techniques. Host cell membranes and schizonts were separated by differential centrifugation following nitrogen decompression. Isolated schizonts were further fractionated into several subcellular compartments. Crossed-immune electrophoresis, against monkey anti-schizont serum, of Triton X-100-solubilized material identified 7 P. knowlesi-specific antigens, of which 4 could be detected only in the host cell membranes. These membranes also contained 3 proteins, with relative molecular masses of 55 000, 65 000 and 90 000 and isoelectric points at pH 4.5, 4.5 and 5.2, respectively, which are lacking in normal membranes. Pulse-chase experiments with (14C)-glucosamine showed that these parasite-induced host cell membrane components are glycoproteins.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown K. N., Brown I. N., Hills L. A. Immunity to malaria. I. Protection against Plasmodium knowlesi shown by monkeys sensitized with drug-suppressed infections or by dead parasites in Freund's adjuvant. Exp Parasitol. 1970 Oct;28(2):304–317. doi: 10.1016/0014-4894(70)90101-3. [DOI] [PubMed] [Google Scholar]
- Li C. C., Hochstadt J. Transport mechanisms in isolated plasma membranes. Nucleoside processing by membrane vesicles from mouse fibroblast cells grown in defined medium. J Biol Chem. 1976 Feb 25;251(4):1175–1180. [PubMed] [Google Scholar]
- Prior R. B., Kreier J. P. Plasmodium berghei freed from host erythrocytes by a continuous-flow ultrasonic system. Exp Parasitol. 1972 Oct;32(2):239–243. doi: 10.1016/0014-4894(72)90030-6. [DOI] [PubMed] [Google Scholar]
- Schmidt-Ullrich R., Wallach D. F. Plasmodium knowlesi-induced antigens in membranes of parasitized rhesus monkey erythrocytes. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4949–4953. doi: 10.1073/pnas.75.10.4949. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Siddiqui W. A. An effective immunization of experimental monkeys against a human malaria parasite, Plasmodium falciparum. Science. 1977 Jul 22;197(4301):388–389. doi: 10.1126/science.406671. [DOI] [PubMed] [Google Scholar]
- Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
- Trigg P. I., Gutteridge W. E. A minimal medium for the growth of Plasmodium knowlesi in dilution cultures. Parasitology. 1971 Feb;62(1):113–123. doi: 10.1017/s0031182000071328. [DOI] [PubMed] [Google Scholar]
- Wallach D. F., Lin P. S. A critical evaluation of plasma membrane fractionation. Biochim Biophys Acta. 1973 Nov;300(3):211–254. doi: 10.1016/0304-4157(73)90005-1. [DOI] [PubMed] [Google Scholar]
- Wallach D. F., Schmidt-Ullrich R. Isolation of plasma membrane vesicles from animal cells. Methods Cell Biol. 1977;15:235–276. doi: 10.1016/s0091-679x(08)60219-8. [DOI] [PubMed] [Google Scholar]