Abstract
A carrier-free electrophoresis apparatus was used to isolate rat erythrocytes parasitized with Plasmodium berghei. The region of high electrophoretic mobility yielded noninfected erythrocytes, whereas that of low electrophoretic mobility yielded erythrocytes infected with viable parasites. Over 98% purity of parasitized erythrocytes was obtained when blood at an advanced stage of parasitaemia was used. Merozoite-rich fractions were also observed. This continuous one-step separation method should provide large numbers of parasitized erythrocytes for use in immunological and biochemical studies of malaria.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HANNIG K. EINE NEUENTWICKLUNG DER TRAEGERFREIEN KONTINUIERLICHEN ELEKTROPHORESE. ZUR TRENNUNG HOCHMOLEKULARER UND GROBDISPERSER TEILCHEN. Hoppe Seylers Z Physiol Chem. 1964;338:211–227. doi: 10.1515/bchm2.1964.338.1-2.211. [DOI] [PubMed] [Google Scholar]
- Haynes J. D., Diggs C. L., Hines F. A., Desjardins R. E. Culture of human malaria parasites Plasmodium falciparum. Nature. 1976 Oct 28;263(5580):767–769. doi: 10.1038/263767a0. [DOI] [PubMed] [Google Scholar]
- Homewood C. A., Neame K. D. A comparison of methods used for the removal of white cells from malaria-infected blood. Ann Trop Med Parasitol. 1976 Jun;70(2):249–251. doi: 10.1080/00034983.1976.11687119. [DOI] [PubMed] [Google Scholar]
- Mitchell G. H., Butcher G. A., Cohen S. A merozoite vaccine effective against Plasmodium knowlesi malaria. Nature. 1974 Nov 22;252(5481):311–313. doi: 10.1038/252311a0. [DOI] [PubMed] [Google Scholar]
- Sato T., Fujii T., Kojima K. Change in electrophoretic mobility of human erythrocyte as the result of membrane shape change in vitro. Physiol Chem Phys. 1975;7(6):523–528. [PubMed] [Google Scholar]
- Stahn R., Maier K. P., Hannig K. A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis. J Cell Biol. 1970 Sep;46(3):576–591. doi: 10.1083/jcb.46.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]