Abstract
Inactivated Lassa virus complement-fixing antigen is normally prepared as a wet frozen antigen, and shipped in dry ice from the Center for Disease Control, Atlanta, Georgia, USA, to other laboratories not equipped for handling live Lassa virus. However, with this antigen there is a considerable loss of potency during shipping. In the search for a more stable antigen, inactivated Lassa antigen was lyophilized and subjected to different storage temperatures. Lyophilization did not significantly affect the CF titre, and the lyophilized antigen remained stable for 15 and 28 days at 25°C and 4°C, respectively.
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Selected References
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- Greiff D., Rightsel W. A. Stabilities of suspensions of viruses after freezing or drying by vacuum sublimation and storage. Cryobiology. 1967 May-Jun;3(6):432–444. doi: 10.1016/s0011-2240(67)80153-6. [DOI] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Wulff H., Lange J. V. Indirect immunofluorescence for the diagnosis of Lassa fever infection. Bull World Health Organ. 1975;52(4-6):429–436. [PMC free article] [PubMed] [Google Scholar]