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. 1979;57(4):649–654.

Attenuated recombinant strains of Vibrio cholerae for oral immunization*

Brahm S Srivastava, V B Sinha, Ranjana Srivastava
PMCID: PMC2395838  PMID: 316742

Abstract

Two attenuated strains of Vibrio cholerae, CD1 and CD3, have been isolated that have remained stable since 1976. These strains are motile, adhere to and multiply in rabbit intestine, and colonize the gut of infant mice for 6-7 days. Both strains are antigenic and provide protection to challenge in the mouse protection test and in the rabbit ileal loop model. Because of their ability to adhere to and colonize the gut, and since they are antigenic, strains CD1 and CD3 have the potentiality of oral vaccines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhaskaran K., Sinha V. B. Transmissible plasmid factors and fertility inhibition in Vibrio cholerae. J Gen Microbiol. 1971 Nov;69(1):89–97. doi: 10.1099/00221287-69-1-89. [DOI] [PubMed] [Google Scholar]
  2. Bhattacharjee J. W., Srivastava B. S. Adherence of wild-type and mutant strains of Vibrio cholerae to normal and immune intestinal tissue. Bull World Health Organ. 1979;57(1):123–128. [PMC free article] [PubMed] [Google Scholar]
  3. Bhattacharjee J. W., Srivastava B. S. Mannose-sensitive haemagglutinins in adherence of Vibrio cholerae eltor to intestine. J Gen Microbiol. 1978 Aug;107(2):407–410. doi: 10.1099/00221287-107-2-407. [DOI] [PubMed] [Google Scholar]
  4. DUTTA N. K., HABBU M. K. Experimental cholera in infant rabbits: a method for chemotherapeutic investigation. Br J Pharmacol Chemother. 1955 Jun;10(2):153–159. doi: 10.1111/j.1476-5381.1955.tb00074.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Das Gupta A., Sinha R., Shrivastava D. L., De S. P., Taneja B. L., Rao M. S., Abou-Gareeb A. H. Controlled field trial of the effectiveness of cholera and cholera El Tor vaccines in Calcutta. Bull World Health Organ. 1967;37(3):371–385. [PMC free article] [PubMed] [Google Scholar]
  6. Guentzel M. N., Berry L. J. Motility as a virulence factor for Vibrio cholerae. Infect Immun. 1975 May;11(5):890–897. doi: 10.1128/iai.11.5.890-897.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmgren J., Svennerholm A. M., Lönnroth I., Fall-Persson M., Markman B., Lundbeck H. Development of improved cholera vaccine based on subunit toxoid. Nature. 1977 Oct 13;269(5629):602–604. doi: 10.1038/269602a0. [DOI] [PubMed] [Google Scholar]
  8. Mel D. M., Terzin A. L., Vuksić L. Studies on vaccination against bacillary dysentery. 3. Effective oral immunization against Shigella flexneri 2a in a field trial. Bull World Health Organ. 1965;32(5):647–655. [PMC free article] [PubMed] [Google Scholar]
  9. Nelson E. T., Clements J. D., Finkelstein R. A. Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies. Infect Immun. 1976 Aug;14(2):527–547. doi: 10.1128/iai.14.2.527-547.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sinha V. B., Bhattacharjee J. W. A streptomycin-sensitive revertant of a streptomycin-dependent strain of Vibrio cholerae. Bull World Health Organ. 1976;53(4):482–484. [PMC free article] [PubMed] [Google Scholar]
  11. Sinha V. B., Srivastava B. S. Attenuation of virulence by P and V plasmids in Vibrio cholerae: strains suitable for oral immunization. Bull World Health Organ. 1979;57(4):643–647. [PMC free article] [PubMed] [Google Scholar]
  12. Vasil M. L., Holmes R. K., Finkelstein R. A. Conjugal transfer of a chromosomal gene determining production of enterotoxin in vibrio cholerae. Science. 1975 Mar 7;187(4179):849–850. doi: 10.1126/science.1114331. [DOI] [PubMed] [Google Scholar]

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