Skip to main content
NCBI home page
Bulletin of the World Health Organization logoLink to Bulletin of the World Health Organization
. 1979;57(6):971–975.

A differential filtration technique for the measurement of schistosome cercarial densities in standing waters

André Theron
PMCID: PMC2395853  PMID: 317025

Abstract

The technique of differential filtration allows the detection of schistosome cercariae, with a recovery greater than 80%, in natural waters with a high concentration of microorganisms. This technique makes use of a new type of monofilament polyamide filter Nytrel-TI, with a pore size of 40 μm, allowing filtration of turbid water without clogging of the filter. A three-level prefiltration column (1000, 500 and 200 μm), which retains organisms larger than cercariae, facilitates reading of the filter. The apparatus used weighs less than 3.5 kg; it requires no power supply and can easily be used in the field.

Full text

PDF
971

Images in this article

974Fig. 3
on p.974

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett P. D., Ellison I. R. A continuous flow centrifuge for testing the presence of Bilharzia cercariae in water. Cent Afr J Med. 1965 Nov;11(11):338–340. [PubMed] [Google Scholar]
  2. Butler J. M., Jr, Ferguson F. F., Palmer J. R. New field device for quantitative recovery of Schistosoma mansoni cercariae. Public Health Rep. 1967 Mar;82(3):250–252. [PMC free article] [PubMed] [Google Scholar]
  3. KLOCK J. W. A method for the direct quantitative recovery of Schistosoma mansoni cercariae from natural waters of Puerto Rico. Bull World Health Organ. 1961;25:738–740. [PMC free article] [PubMed] [Google Scholar]
  4. Olivier L. J. A continuous-flow centrifuge for concentration of schistosome cercariae. Am J Trop Med Hyg. 1966 Nov;15(6):875–881. doi: 10.4269/ajtmh.1966.15.875. [DOI] [PubMed] [Google Scholar]
  5. PESIGAN T. P., HAIRSTON N. G., JAUREGUI J. J., GARCIA E. G., SANTOS A. T., SANTOS B. C., BESA A. A. Studies on Schistosoma japonicum infection in the Philippines. 2. The molluscan host. Bull World Health Organ. 1958;18(4):481–578. [PMC free article] [PubMed] [Google Scholar]
  6. PITCHFORD R. J., VISSER P. S. Results of exposing mice to schistosomiasis by immersion in natural water. Trans R Soc Trop Med Hyg. 1962 Jul;56:294–301. doi: 10.1016/0035-9203(62)90051-2. [DOI] [PubMed] [Google Scholar]
  7. ROWAN W. B. Daily periodicity of Schistosoma mansoni cercariae in Puerto Rican waters. Am J Trop Med Hyg. 1958 Jul;7(4):374–381. doi: 10.4269/ajtmh.1958.7.374. [DOI] [PubMed] [Google Scholar]
  8. ROWAN W. B., GRAM A. L. Relation of water velocity to Schistosoma mansoni infection in mice. Am J Trop Med Hyg. 1959 Nov;8:630–634. doi: 10.4269/ajtmh.1959.8.630. [DOI] [PubMed] [Google Scholar]
  9. Sandt D. G. Direct filtration for recovery of Schistosoma mansoni cercariae in the field. Bull World Health Organ. 1973;48(1):27–34. [PMC free article] [PubMed] [Google Scholar]
  10. Sandt D. G. Evaluation of an overlay technique for the recovery of Schistosoma mansoni cercariae. Bull World Health Organ. 1972;47(1):125–127. [PMC free article] [PubMed] [Google Scholar]
  11. Sandt D. G. Laboratory comparison of four cercaria recovery techniques. Bull World Health Organ. 1973;48(1):35–40. [PMC free article] [PubMed] [Google Scholar]

Articles from Bulletin of the World Health Organization are provided here courtesy of World Health Organization

RESOURCES