Abstract
Serological surveillance of suspected orthopoxvirus infections in man is important for confirming the success of the worldwide smallpox eradication programme. An adsorption radioimmunoassay (RIA) was used to differentiate sera from patients who were naturally infected with human monkeypox or variola virus, and individuals who were immunized with vaccinia virus. The antisera were adsorbed with uninfected chicken chorioallantoic membrane (CAM) and vaccinia-infected CAM before reacting in RIA with vaccinia, monkeypox, and variola antigens. Each serum group showed characteristic patterns of residual antibody activity which made it possible to identify antibody specificities.
When 45 human sera were tested by this method, 71% were identified as having vaccinia, variola, or monkeypox adsorption characteristics, while the remaining 29% could not be identified. Of the identified sera, 9 were characteristic of vaccinia, 8 of variola, and 15 of monkeypox. Six of the 15 monkeypox sera were virologically confirmed monkeypox infections, 6 were suspected monkeypox infections but were not virologically confirmed, and 3 were of unknown aetiology.
The adsorption RIA provides a method of identifying serologically the poxvirus responsible for infection long after the acute phase of illness.
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Selected References
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- Hutchinson H. D., Ziegler D. W. Criteria for preparing, evaluating, and standardizing iodinated globulins for radioimmunoassay procedures. Appl Microbiol. 1974 Dec;28(6):935–942. doi: 10.1128/am.28.6.935-942.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hutchinson H. D., Ziegler D. W., Wells D. E., Nakano J. H. Differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay. Bull World Health Organ. 1977;55(5):613–623. [PMC free article] [PubMed] [Google Scholar]
- Lourie B., Bingham P. G., Evans H. H., Foster S. O., Nakano J. H., Herrmann K. L. Human infection with monkeypox virus: laboratory investigation of six cases in West Africa. Bull World Health Organ. 1972;46(5):633–639. [PMC free article] [PubMed] [Google Scholar]
- Walls H. H., Ziegler D. W., Nakano J. H. A study of the specificities of sequential antisera to variola and monkeypox viruses by radioimmunoassay. Bull World Health Organ. 1980;58(1):131–138. [PMC free article] [PubMed] [Google Scholar]
- Webster R. G. Original antigenic sin in ferrets: the response to sequential infections with influenza viruses. J Immunol. 1966 Aug;97(2):177–183. [PubMed] [Google Scholar]
- Ziegler D. W., Hutchinson H. D., Koplan J. P., Nakano J. H. Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees. J Clin Microbiol. 1975 Mar;1(3):311–317. doi: 10.1128/jcm.1.3.311-317.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]