Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1984 Jan;47(1):31–38. doi: 10.1128/aem.47.1.31-38.1984

Metabolic and ultrastructural response to glucose of two eurytrophic bacteria isolated from seawater at different enriching concentrations.

M Baxter, J M Sieburth
PMCID: PMC239607  PMID: 6696421

Abstract

Two marine bacteria, an Acinetobacter sp. (strain GO1) and a vibrio sp. (strain G1), were isolated by extinction dilution and maintained in natural seawater supplemented with nitrogen, phosphorus, and glucose at 0.01 and 10 mg of glucose carbon per liter above ambient monosaccharide concentrations, respectively. After 3 days in unsupplemented natural seawater, growth in batch culture with glucose supplements was determined by changes in cell numbers and glucose concentration. The exponential growth of the Acinetobacter strain with added glucose was indistinguishable from that in natural seawater alone, whereas that of the Vibrio strain was more rapid in the presence of glucose supplements, suggesting that the Acinetobacter strain preferred the natural organic matter in seawater as a carbon source. The ultrastructure for both isolates was unaffected by glucose supplements during exponential growth, but there were marked changes in stationary-phase cells. The Vibrio strain formed polyphosphate at 10 mg of glucose carbon per liter, whereas poly-beta-hydroxybutyrate formation occurred at 100 mg and became excessive at 1,000 mg, disrupting the cells. In contrast, the Acinetobacter strain elongated at 100 and 1,000 mg of glucose carbon per liter but failed to show poly-beta-hydroxybutyrate formation. The diversity of responses shown here would not have been detected with a single concentration of substrate, often used in the literature to characterize both pure and natural populations of marine bacteria.

Full text

PDF
31

Images in this article

35Image
on p.35
36Image
on p.36

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aragi Y., Taga N., Simidu U. Isolation and distribution of oligotrophic marine bacteria. Can J Microbiol. 1977 Aug;23(8):981–987. doi: 10.1139/m77-146. [DOI] [PubMed] [Google Scholar]
  2. CASSELL E. A. RAPID GRAPHICAL METHOD FOR ESTIMATING THE PRECISION OF DIRECT MICROSCOPIC COUNTING DATA. Appl Microbiol. 1965 May;13:293–296. doi: 10.1128/am.13.3.293-296.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caron D. A., Davis P. G., Madin L. P., Sieburth J. M. Heterotrophic bacteria and bacterivorous protozoa in oceanic macroaggregates. Science. 1982 Nov 19;218(4574):795–797. doi: 10.1126/science.218.4574.795. [DOI] [PubMed] [Google Scholar]
  4. Edwards V. H. The influence of high substrate concentrations on microbial kinetics. Biotechnol Bioeng. 1970 Sep;12(5):679–712. doi: 10.1002/bit.260120504. [DOI] [PubMed] [Google Scholar]
  5. Hamilton R. D., Morgan K. M., Strickland J. D. The glucose uptake kinetics of some marine bacteria. Can J Microbiol. 1966 Oct;12(5):995–1003. doi: 10.1139/m66-134. [DOI] [PubMed] [Google Scholar]
  6. Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jannasch H. W. Growth characteristics of heterotrophic bacteria in seawater. J Bacteriol. 1968 Feb;95(2):722–723. doi: 10.1128/jb.95.2.722-723.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Matin A., Veldkamp H. Physiological basis of the selective advantage of a Spirillum sp. in a carbon-limited environment. J Gen Microbiol. 1978 Apr;105(2):187–197. doi: 10.1099/00221287-105-2-187. [DOI] [PubMed] [Google Scholar]
  9. Mian F. A., Jarman T. R., Righelato R. C. Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa. J Bacteriol. 1978 May;134(2):418–422. doi: 10.1128/jb.134.2.418-422.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Neijssel O. M., Tempest D. W. The regulation of carbohydrate metabolism in Klebsiella aerogenes NCTC 418 organisms, growing in chemostat culture. Arch Microbiol. 1975 Dec 31;106(3):251–258. doi: 10.1007/BF00446531. [DOI] [PubMed] [Google Scholar]
  11. Payne W. J., Wiebe W. J. Growth yield and efficiency in chemosynthetic microorganisms. Annu Rev Microbiol. 1978;32:155–183. doi: 10.1146/annurev.mi.32.100178.001103. [DOI] [PubMed] [Google Scholar]
  12. Sieburth J. M., Willis P. J., Johnson K. M., Burney C. M., Lavoie D. M., Hinga K. R., Caron D. A., French F. W., 3rd, Johnson P. W., Davis P. G. Dissolved organic matter and heterotrophic microneuston in the surface microlayers of the north atlantic. Science. 1976 Dec 24;194(4272):1415–1418. doi: 10.1126/science.194.4272.1415. [DOI] [PubMed] [Google Scholar]
  13. WILKINSON J. F. The extracellualr polysaccharides of bacteria. Bacteriol Rev. 1958 Mar;22(1):46–73. doi: 10.1128/br.22.1.46-73.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Williams A. G., Wimpenny J. W. Extracellular polysaccharide biosynthesis by Pseudomonas NCIB 11264. Studies on precursor-forming enzymes and factors affecting exopolysaccharide production by washed suspensions. J Gen Microbiol. 1980 Jan;116(1):133–141. doi: 10.1099/00221287-116-1-133. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES