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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Stroke. 2008 Jan 31;39(3):967–974. doi: 10.1161/STROKEAHA.107.497412

Figure 2.

Figure 2

Brain slices stained with triphenyltetrazolium chloride 6 days after 1 15-minute period (A) and 3 5-minute periods (B) of middle cerebral artery occlusion (MCAO) show lighter staining in striatum on ischemic side (right) compared with nonischemic side (left), but no asymmetry in staining was observed after two 5-minute periods of MCAO (C). Striatal sections stained with hematoxylin-eosin show some eosinophilic cells with shrunken, pyknotic nuclei (arrows) 6 days after 1 15-minute period of MCAO (D) or 3 5-minute periods of MCAO (E) but intact neurons after 2 5-minute periods of MCAO (F; scale bar=10 μm). Striatal sections stained for TUNEL (brown) and cresyl violet show TUNEL-positive cells 6 days after 1 15-minute period of MCAO (G) or 3 5-minute periods of MCAO (H), but not after 2 5-minute periods of MCAO (I; scale bar=40 μm). All examples in A to I were from isoflurane groups. Similar results were seen in halothane groups. J, Mean±SEM of TUNEL-positive cell density (n=4 in halothane IPC 2X5 minutes and IPC 3X5 minutes; n=3 in other groups) in striatum and cortex (note scale difference). P<0.05 from IPC 2×5 minutes for isoflurane (*) or halothane (#).