Figure 3. Reproducible up-regulation of Mcl-1 gene expression by IL-6.
The blots of three independent RPA experiments were scanned and the values were normalized using the L32-band intensities as internal standards. Results are the values for the six main Bcl-2 family members expressed in XG-6 and XG-13 HMCLs starved of IL-6 and cultured for 6 hours with no cytokine (blank column) or with 2 ng/mL IL-6 (hatched column). Statistical analysis was performed with a Wilcoxon test for pairs, grouping data obtained with XG-6 and XG-13 cells.