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. 1984 Jan;47(1):208–209. doi: 10.1128/aem.47.1.208-209.1984

Improved method for detection of glycosidases in bacterial colonies.

N F Paoni, R L Arroyo
PMCID: PMC239639  PMID: 6364970

Abstract

An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to enable better penetration by chromagenic substrates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Kemp D. J., Cowman A. F. Direct immunoassay for detecting Escherichia coli colonies that contain polypeptides encoded by cloned DNA segments. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4520–4524. doi: 10.1073/pnas.78.7.4520. [DOI] [PMC free article] [PubMed] [Google Scholar]

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