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. 1984 Feb;47(2):253–257. doi: 10.1128/aem.47.2.253-257.1984

Genetic Construction of Lactose-Utilizing Xanthomonas campestris

Patricia M Walsh 1,†,*, Michael J Haas 1, George A Somkuti 1
PMCID: PMC239654  PMID: 16346464

Abstract

Xanthomonas campestris, the producer of xanthan gum, possesses a β-galactosidase of very low specific activity. Plasmid pGC9114 (RP1::Tn951), generated by the transposition of the lactose transposon Tn951 to RP1, was conjugally transferred into XN1, a nalidixic acid-resistant derivative of X. campestris NRRL B-1459S-4L. Transfer occurred on membrane filters and in broth. The β-galactosidase gene of Tn951 was expressed in X. campestris. The specific activity of β-galactosidase in transconjugants was over 200-fold higher than that in XN1, and transconjugants grew as well in lactose-based media as in glucose-based media. The lactose-utilizing transconjugants could potentially be used to produce xanthan gum from cheese whey.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagdasarian M., Lurz R., Rückert B., Franklin F. C., Bagdasarian M. M., Frey J., Timmis K. N. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 1981 Dec;16(1-3):237–247. doi: 10.1016/0378-1119(81)90080-9. [DOI] [PubMed] [Google Scholar]
  2. Baumberg S., Cornelis G., Panagiotakopoulos M., Roberts M. Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas. J Gen Microbiol. 1980 Jul;119(1):257–262. doi: 10.1099/00221287-119-1-257. [DOI] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  4. Cornelis G., Ghosal D., Saedler H. Tn951: a new transposon carrying a lactose operon. Mol Gen Genet. 1978 Apr 6;160(2):215–224. doi: 10.1007/BF00267484. [DOI] [PubMed] [Google Scholar]
  5. Cornelis G. Sequence relationships between plasmids carrying genes for lactose utilization. J Gen Microbiol. 1981 May;124(1):91–97. doi: 10.1099/00221287-124-1-91. [DOI] [PubMed] [Google Scholar]
  6. Frank J. F., Somkuti G. A. General Properties of Beta-Galactosidase of Xanthomonas campestris. Appl Environ Microbiol. 1979 Sep;38(3):554–556. doi: 10.1128/aem.38.3.554-556.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jansson P. E., Kenne L., Lindberg B. Structure of extracellular polysaccharide from Xanthomonas campestris. Carbohydr Res. 1975 Dec;45:275–282. doi: 10.1016/s0008-6215(00)85885-1. [DOI] [PubMed] [Google Scholar]
  9. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  10. Meyer R., Laux R., Boch G., Hinds M., Bayly R., Shapiro J. A. Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range. J Bacteriol. 1982 Oct;152(1):140–150. doi: 10.1128/jb.152.1.140-150.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nagahari K. Deletion plasmids from transformants of Pseudomonas aeruginosa trp cells with the RSF1010-trp hybrid plasmid and high levels of enzyme activity from the gene on the plasmid. J Bacteriol. 1978 Oct;136(1):312–317. doi: 10.1128/jb.136.1.312-317.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nano F. E., Kaplan S. Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides. J Bacteriol. 1982 Nov;152(2):924–927. doi: 10.1128/jb.152.2.924-927.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Souw P., Demain A. L. Nutritional Studies on Xanthan Production by Xanthomonas campestris NRRL B1459. Appl Environ Microbiol. 1979 Jun;37(6):1186–1192. doi: 10.1128/aem.37.6.1186-1192.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Story D. L., Shrader R. E., Theriault L. L., Lumijarvi D. L., Shenoy T. S., Savaiano D. A., Shaffer R. H., Ho C. Y., Clifford A. J. Effects of dietary protein, adenine, and allopurinol on growth and metabolism of rats. J Nutr. 1977 Jun;107(6):1044–1052. doi: 10.1093/jn/107.6.1044. [DOI] [PubMed] [Google Scholar]

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