Abstract
Adhesion of Colletotrichum lindemuthianum spores to Phaseolus vulgaris hypocotyls and to polystyrene was inhibited by the respiratory inhibitors sodium azide and antimycin A, indicating a requirement for metabolic activity in adhesion. Various commercial proteins and Tween 80 also reduced adhesion to both surfaces. Binding was enhanced by the presence of salts: sodium, potassium, calcium, and magnesium chlorides were equally effective. The removal of surface wax from hypocotyls by chloroform treatment greatly reduced their subsequent ability to bind spores. The results suggest a similar mechanism for spore adhesion to the plant surface and to polystyrene, involving purely physical surface properties rather than group-specific binding sites.
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Selected References
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- Baier R. E., Shafrin E. G., Zisman W. A. Adhesion: mechanisms that assist or impede it. Science. 1968 Dec 20;162(3860):1360–1368. doi: 10.1126/science.162.3860.1360. [DOI] [PubMed] [Google Scholar]
- Fletcher M. The effects of proteins on bacterial attachment to polystyrene. J Gen Microbiol. 1976 Jun;94(2):400–404. doi: 10.1099/00221287-94-2-400. [DOI] [PubMed] [Google Scholar]
- Marshall K. C., Cruickshank R. H. Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Arch Mikrobiol. 1973 Apr 8;91(1):29–40. doi: 10.1007/BF00409536. [DOI] [PubMed] [Google Scholar]
- Perkins F. O. The ultrastructure of holdfasts, "rhizoids", and "slime tracks" in Thraustochytriaceous fungi and Labyrinthula spp. Arch Mikrobiol. 1972;84(2):95–118. doi: 10.1007/BF00412431. [DOI] [PubMed] [Google Scholar]
- Rosenberg M. Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity. Appl Environ Microbiol. 1981 Aug;42(2):375–377. doi: 10.1128/aem.42.2.375-377.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sing V. O., Bartnicki-Garcia S. Adhesion of Phytophthora palmivora zoospores: electron microscopy of cell attachment and cyst wall fibril formation. J Cell Sci. 1975 Jun;18(1):123–132. doi: 10.1242/jcs.18.1.123. [DOI] [PubMed] [Google Scholar]