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. 1984 Apr;47(4):699–703. doi: 10.1128/aem.47.4.699-703.1984

Properties of Alginate Lyases from Marine Bacteria

R Scott Doubet 1, Ralph S Quatrano 1,*
PMCID: PMC239751  PMID: 16346509

Abstract

Alginate lyases (EC 4.2.2.3) from two marine bacteria were isolated and partially characterized. A cell-bound lyase from isolate A3 had a molecular weight of approximately 100,000 and cleaved mannuronate blocks, apparently in an exo manner. A lyase recovered from the culture medium of isolate W3 was soluble in saturated ammonium sulfate, cleaved guluronate blocks, apparently in an endo manner, and had a molecular weight of 35,000. The thiobarbiturate test and urea-polyacrylamide gel electrophoresis were used to determine substrate specificity and mode of substrate cleavage by the enzymes.

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Selected References

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