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. 1984 Apr;47(4):808–813. doi: 10.1128/aem.47.4.808-813.1984

Effects of Temperature on Methanogenesis in a Thermophilic (58°C) Anaerobic Digestor

S H Zinder 1,*, T Anguish 1, S C Cardwell 1
PMCID: PMC239768  PMID: 16346519

Abstract

The short-term effects of temperature on methanogenesis from acetate or CO2 in a thermophilic (58°C) anaerobic digestor were studied by incubating digestor sludge at different temperatures with 14C-labeled methane precursors (14CH3COO− or 14CO2). During a period when Methanosarcina sp. was numerous in the sludge, methanogenesis from acetate was optimal at 55 to 60°C and was completely inhibited at 65°C. A Methanosarcina culture isolated from the digestor grew optimally on acetate at 55 to 58°C and did not grow or produce methane at 65°C. An accidental shift of digestor temperature from 58 to 64°C during this period caused a sharp decrease in gas production and a large increase in acetate concentration within 24 h, indicating that the aceticlastic methanogens in the digestor were the population most susceptible to this temperature increase. During a later period when Methanothrix sp. was numerous in the digestor, methanogenesis from 14CH3COO− was optimal at 65°C and completely inhibited at 75°C. A partially purified Methanothrix enrichment culture derived from the digestor had a maximum growth temperature near 70°C. Methanogenesis from 14CO2 in the sludge was optimal at 65°C and still proceeded at 75°C. A CO2-reducing Methanobacterium sp. isolated from the digestor was capable of methanogenesis at 75°C. During the period when Methanothix sp. was apparently dominant, sludge incubated for 24 h at 65°C produced more methane than sludge incubated at 60°C, and no acetate accumulated at 65°C. Methanogenesis was severely inhibited in sludge incubated at 70°C, but since neither acetate nor H2 accumulated, production of these methanogenic substrates by fermentative bacteria was probably the most temperature-sensitive process. Thus, there was a correlation between digestor performance at different temperatures and responses to temperature by cultures of methanogens believed to play important roles in the digestor.

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Selected References

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