Abstract
In this report I describe a modification of the gelatin-matrix method for determining the percentages of bacterial cells having active electron transport systems. This modification is one-step fluorochroming followed by use of an antioxidant solution instead of the secondary gelatin layer. The modified technique is more successfully applicable to water samples containing high densities of flocculant particulate matter, owing to the brighter green fluorescence of the cells, which contrasts with the red-orange fluorescence of the floc.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bright J. J., Fletcher M. Amino Acid assimilation and electron transport system activity in attached and free-living marine bacteria. Appl Environ Microbiol. 1983 Mar;45(3):818–825. doi: 10.1128/aem.45.3.818-825.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giloh H., Sedat J. W. Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science. 1982 Sep 24;217(4566):1252–1255. doi: 10.1126/science.7112126. [DOI] [PubMed] [Google Scholar]
- Harvey R. W., Young L. Y. Enumeration of particle-bound and unattached respiring bacteria in the salt marsh environment. Appl Environ Microbiol. 1980 Jul;40(1):156–160. doi: 10.1128/aem.40.1.156-160.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kirchman D., Mitchell R. Contribution of particle-bound bacteria to total microheterotrophic activity in five ponds and two marshes. Appl Environ Microbiol. 1982 Jan;43(1):200–209. doi: 10.1128/aem.43.1.200-209.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newell S. Y., Christian R. R. Frequency of dividing cells as an estimator of bacterial productivity. Appl Environ Microbiol. 1981 Jul;42(1):23–31. doi: 10.1128/aem.42.1.23-31.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tabor P. S., Neihof R. A. Improved method for determination of respiring individual microorganisms in natural waters. Appl Environ Microbiol. 1982 Jun;43(6):1249–1255. doi: 10.1128/aem.43.6.1249-1255.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zimmermann R., Iturriaga R., Becker-Birck J. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol. 1978 Dec;36(6):926–935. doi: 10.1128/aem.36.6.926-935.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]