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. 1991 Feb;65(2):1007–1012. doi: 10.1128/jvi.65.2.1007-1012.1991

Characterization of the antigenic domains of the major core protein (p26) of equine infectious anemia virus.

Y H Chong 1, S L Payne 1, C J Issel 1, R C Montelaro 1, K E Rushlow 1
PMCID: PMC239849  PMID: 1702839

Abstract

A panel of recombinant trpLE-gag fusion proteins and synthetic peptides was used in Western immunoblot and enzyme-linked immunosorbent assays to identify segments of the major core protein (p26) of equine infectious anemia virus that are antigenic in horses during experimental and natural infections with the virus. The predominant humoral immune response was directed toward a highly immunogenic domain composed of 83 amino acids from the carboxy terminus of p26. The observed immunogenicity of p26 resembled that reported for p24 of human immunodeficiency virus type 1, suggesting the conservation of structural motifs in the lentiviral core proteins which are responsible for their observed immunogenicity during persistent lentivirus infections.

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Selected References

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  1. Argos P. A possible homology between immunodeficiency virus p24 core protein and picornaviral VP2 coat protein: prediction of HIV p24 antigenic sites. EMBO J. 1989 Mar;8(3):779–785. doi: 10.1002/j.1460-2075.1989.tb03438.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball J. M., Rao V. S., Robey W. G., Issel C. J., Montelaro R. C. Lentivirus antigen purification and characterization: isolation of equine infectious anemia virus gag and env proteins in one step by reverse phase HPLC and application to human immunodeficiency virus glycoproteins. J Virol Methods. 1988 Mar-Apr;19(3-4):265–277. doi: 10.1016/0166-0934(88)90021-3. [DOI] [PubMed] [Google Scholar]
  3. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  4. Coates A. R., Cookson J., Barton G. J., Zvelebil M. J., Sternberg M. J. AIDS vaccine predictions. Nature. 1987 Apr 9;326(6113):549–550. doi: 10.1038/326549c0. [DOI] [PubMed] [Google Scholar]
  5. Coggins L., Norcross N. L., Nusbaum S. R. Diagnosis of equine infectious anemia by immunodiffusion test. Am J Vet Res. 1972 Jan;33(1):11–18. [PubMed] [Google Scholar]
  6. Ferns R. B., Partridge J. C., Spence R. P., Hunt N., Tedder R. S. Epitope location of 13 anti-gag HIV-1 monoclonal antibodies using oligopeptides and their cross reactivity with HIV-2. AIDS. 1989 Dec;3(12):829–834. doi: 10.1097/00002030-198912000-00008. [DOI] [PubMed] [Google Scholar]
  7. Gerencer M., Valpotić I., Jukić B., Tomasković M., Basić I. Qualitative analyses of cellular immune functions in equine infectious anemia show homology with AIDS. Arch Virol. 1989;104(3-4):249–257. doi: 10.1007/BF01315547. [DOI] [PubMed] [Google Scholar]
  8. Hussain K. A., Issel C. J., Schnorr K. L., Rwambo P. M., Montelaro R. C. Antigenic analysis of equine infectious anemia virus (EIAV) variants by using monoclonal antibodies: epitopes of glycoprotein gp90 of EIAV stimulate neutralizing antibodies. J Virol. 1987 Oct;61(10):2956–2961. doi: 10.1128/jvi.61.10.2956-2961.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koup R. A., Sullivan J. L., Levine P. H., Brewster F., Mahr A., Mazzara G., McKenzie S., Panicali D. Antigenic specificity of antibody-dependent cell-mediated cytotoxicity directed against human immunodeficiency virus in antibody-positive sera. J Virol. 1989 Feb;63(2):584–590. doi: 10.1128/jvi.63.2.584-590.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Malmquist W. A., Barnett D., Becvar C. S. Production of equine infectious anemia antigen in a persistently infected cell line. Arch Gesamte Virusforsch. 1973;42(4):361–370. doi: 10.1007/BF01250717. [DOI] [PubMed] [Google Scholar]
  12. Miozzari G. F., Yanofsky C. Translation of the leader region of the Escherichia coli tryptophan operon. J Bacteriol. 1978 Mar;133(3):1457–1466. doi: 10.1128/jb.133.3.1457-1466.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Montelaro R. C., West M., Issel C. J. Antigenic reactivity of the major glycoprotein of equine infectious anemia virus, a retrovirus. Virology. 1984 Jul 30;136(2):368–374. doi: 10.1016/0042-6822(84)90173-9. [DOI] [PubMed] [Google Scholar]
  14. Nixon D. F., Townsend A. R., Elvin J. G., Rizza C. R., Gallwey J., McMichael A. J. HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides. Nature. 1988 Dec 1;336(6198):484–487. doi: 10.1038/336484a0. [DOI] [PubMed] [Google Scholar]
  15. Orrego A., Issel C. J., Montelaro R. C., Adams W. V., Jr Virulence and in vitro growth of a cell-adapted strain of equine infectious anemia virus after serial passage in ponies. Am J Vet Res. 1982 Sep;43(9):1556–1560. [PubMed] [Google Scholar]
  16. Parker J. M., Guo D., Hodges R. S. New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry. 1986 Sep 23;25(19):5425–5432. doi: 10.1021/bi00367a013. [DOI] [PubMed] [Google Scholar]
  17. Payne S. L., Rushlow K., Dhruva B. R., Issel C. J., Montelaro R. C. Localization of conserved and variable antigenic domains of equine infectious anemia virus envelope glycoproteins using recombinant env-encoded protein fragments produced in Escherichia coli. Virology. 1989 Oct;172(2):609–615. doi: 10.1016/0042-6822(89)90203-1. [DOI] [PubMed] [Google Scholar]
  18. Payne S. L., Salinovich O., Nauman S. M., Issel C. J., Montelaro R. C. Course and extent of variation of equine infectious anemia virus during parallel persistent infections. J Virol. 1987 Apr;61(4):1266–1270. doi: 10.1128/jvi.61.4.1266-1270.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ratner L. Measurement of human immunodeficiency virus load and its relation to disease progression. AIDS Res Hum Retroviruses. 1989 Apr;5(2):115–119. doi: 10.1089/aid.1989.5.115. [DOI] [PubMed] [Google Scholar]
  20. Salinovich O., Payne S. L., Montelaro R. C., Hussain K. A., Issel C. J., Schnorr K. L. Rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection. J Virol. 1986 Jan;57(1):71–80. doi: 10.1128/jvi.57.1.71-80.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shane B. S., Issel C. J., Montelaro R. C. Enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody. J Clin Microbiol. 1984 Mar;19(3):351–355. doi: 10.1128/jcm.19.3.351-355.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stephens R. M., Casey J. W., Rice N. R. Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science. 1986 Feb 7;231(4738):589–594. doi: 10.1126/science.3003905. [DOI] [PubMed] [Google Scholar]
  23. Walker B. D., Plata F. Cytotoxic T lymphocytes against HIV. AIDS. 1990 Mar;4(3):177–184. doi: 10.1097/00002030-199003000-00001. [DOI] [PubMed] [Google Scholar]
  24. Windheuser M. G., Tegtmeier G. E., Wood C. Use of TrpE/Gag fusion proteins to characterize immunoreactive domains on the human immunodeficiency virus type 1 core protein. J Virol. 1989 Sep;63(9):4064–4068. doi: 10.1128/jvi.63.9.4064-4068.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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