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. 1991 Mar;65(3):1623–1627. doi: 10.1128/jvi.65.3.1623-1627.1991

Biogenesis of type I cytopathic vacuoles in Semliki Forest virus-infected BHK cells.

J Peränen 1, L Kääriäinen 1
PMCID: PMC239950  PMID: 1825342

Abstract

We investigated the biogenesis of type I cytopathic vacuoles (CPVIs) in Semliki Forest virus (SFV)-infected cells by immunofluorescence and electron microscopy. By using the ts1 mutant of SFV at the restrictive temperature to avoid superinfection, we showed that the multiplicity of infection affects the time of appearance but not the number of CPVIs in a cell. Formation of CPVIs did not require incoming virus particles, because they were found in BHK cells transfected with infectious RNA from the SFV prototype strain or ts1 mutant. When the SFV gene for nsP3 was expressed alone in BHK cells, the nsP3 protein was localized to numerous vesiclelike structures and large vacuoles. The nsP3 protein may function as an anchoring protein for the RNA replication complex of SFV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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